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Dietary arginine level and /sup 3/H-thymidine incorporation into DNA in normal and regenerating liver of weanling rats

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:7134759
; ;

Male weanling Sprague-Dawley rats were fed graded amounts of arginine (0.25% - 2%) in a crystalline L-amino acid diet for 10 days. Feed intake was decreased only in the rats receiving 0.25% Arg in the diet. Weight gain and growth efficiency were lower in the rats receiving 0.25% and 0.5% dietary arginine. Orotic acid excretion was elevated 400 fold in the rats receiving less than 1% dietary arginine. Liver weights were not significantly different among groups. DNA concentration/g liver was 10% lower in the rats receiving 0.25% dietary arginine. Rats receiving less than 1% arginine had 17% less RNA/g liver and 77% less /sup 3/H-thymidine incorporation into liver DNA. After partial hepatectomy (PH) /sup 3/H-thymidine incorporation into liver DNA was increased by 23% on rats receiving 0.5 dietary arginine compared to sham operated controls (SOC) receiving the same diet. Incorporation of /sup 3/H-thymidine was unchanged in PH rats as compared with SOC rats receiving 0.25% Arg. These data indicate that at least 1% of dietary arginine is required to maintain normal protein metabolism in growing male rats. There were no significant differences in any of the parameters measured when dietary Arg was 1% or 2% of the diet.

Research Organization:
Hebrew Univ. of Jerusalem, Israel
OSTI ID:
7134759
Report Number(s):
CONF-8604222-
Journal Information:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:4; ISSN FEPRA
Country of Publication:
United States
Language:
English

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Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
ARGININE
AZINES
BIOLOGICAL RECOVERY
BIOLOGICAL REGENERATION
BODY
CARBOXYLIC ACIDS
DIET
DIGESTIVE SYSTEM
DNA
GLANDS
HEPATECTOMY
HETEROCYCLIC COMPOUNDS
INTAKE
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIVER
MAMMALS
MEDICINE
METABOLISM
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
PYRIMIDINES
RATS
RECOVERY
RIBOSIDES
RODENTS
SURGERY
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES