Dual isotope Schilling test for measuring absorption of food-bound and free vitamin B12 simultaneously
A prototype food-bound vitamin B12 (food-B12) absorption test has been developed in which /sup 57/Co-B12 was incorporated in vitro into egg yolk (yolk-B12) and served to volunteers in 50-g cooked portions together with toast and coffee for breakfast. Six hours later, 1 mg nonlabeled B12 was given intramuscularly and 24-hour urine was collected for radioactivity measurement. In separate tests, the absorption of yolk-B12 and crystalline /sup 57/Co-B12 was equally poor in patients with pernicious anemia. However, in patients with simple gastric achlorhydria and those who had undergone gastric surgery, the assimilation of yolk-B12 was impaired greatly, whereas the absorption of crystalline radio-B12 was normal. Egg yolk labeled with /sup 58/Co-B12 was administered together with crystalline /sup 57/Co-B12 in a dual isotope test with results similar to those obtained when the tests were prepared separately. This yolk-/sup 58/Co-B12 test with its ability to detect malabsorption of food-B12 may be considered as an addition to the first part of the Schilling test.
- OSTI ID:
- 7133750
- Journal Information:
- Am. J. Clin. Pathol.; (United States), Journal Name: Am. J. Clin. Pathol.; (United States) Vol. 80:4; ISSN AJCPA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION
ANIMALS
BETA DECAY RADIOISOTOPES
COBALT 57
COBALT ISOTOPES
DAYS LIVING RADIOISOTOPES
DRUGS
DUAL-ISOTOPE SUBTRACTION TECHNIQUE
ELECTRON CAPTURE RADIOISOTOPES
FOOD
HEMATINICS
HEMATOLOGIC AGENTS
INGESTION
INTAKE
INTERMEDIATE MASS NUCLEI
INTESTINAL ABSORPTION
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MAN
MEASURING METHODS
NUCLEI
ODD-EVEN NUCLEI
PRIMATES
RADIOISOTOPES
TRACER TECHNIQUES
UPTAKE
VERTEBRATES
VITAMIN B GROUP
VITAMIN B-12
VITAMINS