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14C-Cobalamin Absorption from Endogenously Labeled Chicken Eggs Assessed in Humans Using Accelerator Mass Spectrometry

Journal Article · · Nutrients
DOI:https://doi.org/10.3390/nu11092148· OSTI ID:1562375
 [1];  [2];  [2];  [3];  [2];  [4];  [1]
  1. US Dept. of Agriculture (USDA), Davis, CA (United States). ARS Western Human Nutrition Research Center
  2. Univ. of California, Davis, CA (United States)
  3. Rutgers Univ., New Brunswick, NJ (United States)
  4. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
+ Show Author Affiliations

Traditionally, the bioavailability of vitamin B-12 (B12) from in vivo labeled foods was determined by labeling the vitamin with radiocobalt (57Co, 58Co or 60Co). This required use of penetrating radioactivity and sometimes used higher doses of B12 than the physiological limit of B12 absorption. The aim of this study was to determine the bioavailability and absorbed B12 from chicken eggs endogenously labeled with 14C-B12 using accelerator mass spectrometry (AMS). 14C-B12 was injected intramuscularly into hens to produce eggs enriched in vivo with the 14C labeled vitamin. The eggs, which provided 1.4 to 2.6 μg of B12 (~1.1 kBq) per serving, were scrambled, cooked and fed to 10 human volunteers. Baseline and post-ingestion blood, urine and stool samples were collected over a one-week period and assessed for 14C-B12 content using AMS. Bioavailability ranged from 13.2 to 57.7% (mean 30.2 ± 16.4%). Difference among subjects was explained by dose of B12, with percent bioavailability from 2.6 μg only half that from 1.4 μg. The total amount of B12 absorbed was limited to 0.5–0.8 μg (mean 0.55 ± 0.19 μg B12) and was relatively unaffected by the amount consumed. The use of 14C-B12 offers the only currently available method for quantifying B12 absorption in humans, including food cobalamin absorption. An egg is confirmed as a good source of B12, supplying approximately 20% of the average adult daily requirement (RDA for adults = 2.4 μg/day).

Research Organization:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
Sponsoring Organization:
USDOE National Nuclear Security Administration (NNSA); National Institute of Health (NIH)
Grant/Contract Number:
AC52-07NA27344
OSTI ID:
1562375
Report Number(s):
LLNL-JRNL--757858; 945835
Journal Information:
Nutrients, Journal Name: Nutrients Journal Issue: 9 Vol. 11; ISSN 2072-6643; ISSN NUTRHU
Publisher:
MDPICopyright Statement
Country of Publication:
United States
Language:
English

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Figures / Tables (14)

Figure 1(p. 6)figure Figure 1
Table 1(p. 7)table Table 1
Figure 2(p. 9)figure Figure 2
Figure 3(p. 9)figure Figure 3
Table 2(p. 10)table Table 2
Figure 4(p. 11)figure Figure 4
Figure 5(p. 11)figure Figure 5
Figure 6(p. 11)figure Figure 6
Figure 7(p. 12)figure Figure 7
Figure A1(p. 15)figure Figure A1
Figure A2(p. 16)figure Figure A2
Table A1(p. 16)table Table A1
Figure A3(p. 17)figure Figure A3
Figure A4(p. 17)figure Figure A4

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Related Subjects

60 APPLIED LIFE SCIENCES
accelerator mass spectrometry
bioavailability
cobalamin
eggs
endogenous label
human
vitamin B12