Supercritical fluid chromatography with small particle diameter packed columns

Journal Article · Thu Apr 01 04:00:00 EST 1982 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00241a032· OSTI ID:7119611

Gere, D R ^[1]; Board, R; McManigill, D

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1. Hewlett-Packard Co., Avondale, PA

Supercritical fluid chromatography is carried out with 10, 5, and 3 ..mu..m particle diameter packed columns. Reduced plate heights between 2.0 and 3.0 are achieved in the region of the van Deemter minimum. Using polycyclic aromatic hydrocarbons as probe molecules, the relationship of density of supercritical carbon dioxide to log capacity factor was studied between 32 and 100/sup 0/C. The efficiency of the columns appears to improve as the particle diameter becomes smaller. With a UV detector, minimum detectable quantities are in the range of 1 ng mass injected. Resolution per unit time is 5-10 times better than HPLC with the same columns due to more favorable diffusivity in supercritical fluids. Usable regions of temperature, pressure, density, and flow rate are defined. 9 figures.

• OSTI ID: 7119611
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 54:4; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400104 -- Spectral Procedures-- (-1987)
400105* -- Separation Procedures
AROMATICS
CALIBRATION
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CHALCOGENIDES
CHEMICAL ANALYSIS
CHROMATOGRAPHY
COLUMN PACKING
COMPRESSIBLE FLOW
DATA
DENSITY
EFFICIENCY
EXPERIMENTAL DATA
EXTRACTION APPARATUSES
EXTRACTION COLUMNS
FLOW RATE
FLOWMETERS
FLUID FLOW
FLUIDS
GASES
HIGH PRESSURE
HYDROCARBONS
INFORMATION
ISOTHERM
MEASURING INSTRUMENTS
MEDIUM TEMPERATURE
METERS
NUMERICAL DATA
OPERATION
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PACKINGS
PARAMETRIC ANALYSIS
PARTICLE SIZE
PHYSICAL PROPERTIES
POLYCYCLIC AROMATIC HYDROCARBONS
PRESSURE DEPENDENCE
QUANTITATIVE CHEMICAL ANALYSIS
SEPARATION EQUIPMENT
SEPARATION PROCESSES
SIZE
SUPERCRITICAL FLUID CHROMATOGRAPHY
TEMPERATURE DEPENDENCE