Supercritical fluid chromatography

Journal Article · Fri Oct 21 04:00:00 EDT 1983 · Science (Washington, D.C.); (United States)

DOI:https://doi.org/10.1126/science.6414083· OSTI ID:5313278

Gere, D R

Chromatographic separations with a supercritical fluid as the mobile phase were suggested more than 20 years ago. Availability of commercial hardware makes this technique more widely usable today. Many separations by this method are now carried out with supercritical carbon dioxide as the mobile phase and packed liquid-chromatography columns as the stationary phase. Although carbon dioxide has many practical advantages, including its near-ambient critical temperature and minimal interference with spectrometric detection, the use of other supercritical fluids or addition of modifiers to carbon dioxide may extend the applications of this technique. Some mixtures that are difficult to analyze by other chromatographic methods may be susceptible to separation by supercritical fluid chromatography. Mixtures that have been separated with supercritical carbon dioxide include resin acids with the empirical formula C/sub 20/H/sub 30/O/sub 2/ and ubiquinones from bacterial cell wall extracts of Legionella pneumophila. 60 refs., 8 figs.

• Research Organization: Hewlett-Packard, Avondale, PA
• OSTI ID: 5313278
• Journal Information: Science (Washington, D.C.); (United States), Journal Name: Science (Washington, D.C.); (United States) Vol. 222:4621; ISSN SCIEA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400105* -- Separation Procedures
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CHALCOGENIDES
CHROMATOGRAPHY
GAS CHROMATOGRAPHY
OXIDES
OXYGEN COMPOUNDS
PRESSURE DEPENDENCE
SEPARATION PROCESSES
SOLVENT PROPERTIES
SUPERCRITICAL FLUID CHROMATOGRAPHY