New acute transforming feline retovirus with fms homology specifies a C-terminally truncated version of the c-fms protein that is different from SM-feline sarcoma virus v-fms protein

Journal Article · Wed Oct 01 00:00:00 EDT 1986 · J. Virol.; (United States)

OSTI ID:7118672

Besmer, P; Lader, E; George, P C; Bergold, P J; Qui, F; Zuckerman, E E; Hardy, W D

The HZ5-feline sarcoma virus (FeSV) is a new acute transforming feline retrovirus which was isolated from a multicentric fibrosarcoma of a domestic cat. The HZ5-FeSV transforms fibroblasts in vitro and is replication defective. A biologically active integrated HZ5-FeSV provirus was molecularly cloned from cellular DNA of HZ5-FeSV-infected FRE-3A rat cells. The HZ5-FeSV has oncogene homology with the fms sequences of the SM-FeSV. The genome organization of the 8.6-kilobase HZ5-FeSV provirus is 5' ..delta..gag-fms-..delta..pol-..delta..env 3'. The HZ5- and SM-FeSVs display indistinguishable in vitro transformation characteristics, and the structures of the gag-fms transforming genes in the two viruses are very similar. In the HZ5-FeSV and the SM-FeSV, identical c-fms and feline leukemia virus p10 sequences form the 5' gag-fms junction. With regard to v-fms the two viruses are homologous up to 11 amino acids before the C terminus of the SM-FeSV v-fms protein. In HZ5-FeSV a segment of 362 nucleotides then follows before the 3' recombination site with feline leukemia virus pol. The new 3' v-fms sequence encodes 27 amino acids before reaching a TGA termination signal. The relationship of this sequence with the recently characterized human c-fms sequence has been examined. The 3' HZ5-FeSV v-fms sequence is homologous with 3' c-fms sequences. A frameshift mutation (11-base-pair deletion) was found in the C-terminal fms coding sequence of the HZ5-FeSV. As a result, the HZ5-FeSV v-fms protein is predicted to be a C-terminally truncated version of c-fms. This frameshift mutation may determine the oncogenic properties of v-fms in the HZ5-FeSV.

• Research Organization: Memorial-Sloan Kettering Cancer Center, New York, NY
• OSTI ID: 7118672
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 60:1; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CATS
CELL CONSTITUENTS
CELL TRANSFORMATIONS
CLONING
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
DNA SEQUENCING
DNA-CLONING
FIBROBLASTS
FIBROSARCOMAS
HYBRIDIZATION
ISOTOPE APPLICATIONS
ISOTOPES
LEUKEMIA VIRUSES
LIGHT NUCLEI
MAMMALS
MICROORGANISMS
NEOPLASMS
NUCLEI
ODD-ODD NUCLEI
ONCOGENIC TRANSFORMATIONS
ONCOGENIC VIRUSES
PARASITES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLASMIDS
RADIOISOTOPES
RATS
RODENTS
SARCOMAS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
TRACER TECHNIQUES
VERTEBRATES
VIRUSES