Analysis of the primary structure of the long terminal repeat and the gag and pol genes of the human spumaretrovirus
- Deutsches Krebsforschungszentrum, Heidelberg (West Germany)
- Universitaet Heidelberg (West Germany)
The nucleotide sequence of the human spumaretrovirus (HSRV) genome was determined. The 5{prime} long terminal repeat region was analyzed by strong stop cDNA synthesis and S1 nuclease mapping. The length of the RU5 region was determined and found to be 346 nucleotides long. The 5{prime} long terminal repeat is 1,123 base pairs long and is bound by an 18-base-pair primer-binding site complementary to the 3{prime} end of mammalian lysine-1,2-specific tRNA. Open reading frames for gag and pol genes were identified. The pol gene overlaps the gag gene and is postulated to be synthesized as a gag/pol precursor via translational frameshifting analogous to that of Rous sarcoma virus, with 7 nucleotides immediately upstream of the termination codons of gag conserved between the two viral genomes. The HSRV pol gene is 2,730 nucleotides long, and its deduced protein sequence is readily subdivided into three well-conserved domains, the reverse transcriptase, the RNase H, and the integrase. Although the degree of homology of the HSRV reverse transcriptase domain is highest to that of murine leukemia virus, the HSRV genomic organization is more similar to that of human and simian immunodeficiency viruses. The data justify classifying the spumaretroviruses as a third subfamily of Retroviridae.
- OSTI ID:
- 6533382
- Journal Information:
- Journal of Virology; (USA), Journal Name: Journal of Virology; (USA) Vol. 62:5; ISSN JOVIA; ISSN 0022-538X
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DNA
DNA POLYMERASES
DNA SEQUENCING
EMBRYONIC CELLS
ENZYMES
ESTERASES
FIBROBLASTS
GENES
GENETIC MAPPING
HYDROLASES
ISOTOPES
LIGHT NUCLEI
LUNGS
MAMMALS
MAN
MAPPING
MICROORGANISMS
NUCLEASES
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
NUCLEOTIDYLTRANSFERASES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PARASITES
PHOSPHODIESTERASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PRIMATES
RADIOISOTOPES
RECOMBINANT DNA
RESPIRATORY SYSTEM
RNA
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
TRANSFER RNA
TRANSFERASES
VERTEBRATES
VIRUSES