Myristylation of gag-onc fusion proteins in mammalian transforming retroviruses
Four cell lines producing transforming proteins encoded by three mammalian oncogenes (fes, abl, and ras) were investigated for incorporation of (/sup 3/H)myristate into gag-onc fusion proteins. Using 5-min pulse-labelings, fusion proteins of Abelson murine leukemia virus, Gardner-Arnstein strain of feline sarcoma virus (FeSV), and Snyder-Theilen strain of FeSV were shown to be myristylated. In a 4-hr pulse, p29gag-ras of rat sarcoma virus (RaSV) was also shown to incorporate radiolabel. The fatty acid was recovered from this labeled protein by acid hydrolysis, and identified by reverse-phase thin-layer chromatography to be (/sup 3/H)myristic acid. The results indicate that substitution of viral gag sequences by cellular oncogene sequences does not abolish their ability to become post-translationally modified by this long chain fatty acid. It is assumed that in the fusion proteins the myristyl moiety is linked through an amide linkage to the amino-terminal glycine as previously found for several retroviral gag precursor polyproteins. The possible role of myristylation of transforming proteins is discussed.
- Research Organization:
- Laboratory of Molecular Virology and Carcinogenesis, NCI-Frederick Cancer Research Facility, Maryland
- OSTI ID:
- 6833019
- Journal Information:
- Virology; (United States), Journal Name: Virology; (United States) Vol. 133:2; ISSN VIRLA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
BIOCHEMISTRY
CARBOXYLIC ACIDS
CELL CULTURES
CHEMISTRY
CHROMATOGRAPHY
LABELLED COMPOUNDS
METABOLISM
MICROORGANISMS
MOLECULAR STRUCTURE
MONOCARBOXYLIC ACIDS
ONCOGENIC TRANSFORMATIONS
ONCOGENIC VIRUSES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PARASITES
SEPARATION PROCESSES
THIN-LAYER CHROMATOGRAPHY
TRITIUM COMPOUNDS
VIRUSES