Angiogenin activates phospholipase C and elicits a rapid incorporation of fatty acid into cholesterol esters in vascular smooth muscle cells

Journal Article · Mon Jan 08 23:00:00 EST 1990 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00453a031· OSTI ID:7118504

Moore, F; Riordan, J F ^[1]

---

1. Harvard Medical School, Boston, MA (USA)

Angiogenin activates the phosphoinositide-specific phospholipase C (PLC) in cultured rat aortic smooth muscle cells to yield a transient (30 s) peak of 1,2-diacylglycerol (DG) and inositol trisphosphate. Within 1 min, the DG level falls below that of the control and remains so for at least 20 min. A transient increase in monoacylglycerol indicates that depletion of DG may be the consequence of hydrolysis by DG lipase. In addition to these changes in second messengers, a rapid increase in incorporating of radiolabeled tracer into cellular cholesterol esters is observed. Stimulated cholesterol ester labeling is inhibited by preincubation with either the DG lipase inhibitor RHC 80267 or the acyl coenzyme A:cholesterol acyltransferase inhibitor Sandoz 58035. Cells prelabeled with ({sup 3}H)arachidonate show a sustained increase in labeling of cholesterol esters following exposure to angiogenin. In contrast, cells prelabeled with ({sup 3}H)oleate show only a transient elevation that returns to the basal level by 5 min. This suggests initial cholesterol esterification by oleate followed by arachidonate that is released by stimulation of the PLC/DG lipase pathway.

• OSTI ID: 7118504
• Journal Information: Biochemistry; (USA), Journal Name: Biochemistry; (USA) Vol. 29:1; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AORTA
ARACHIDONIC ACID
ARTERIES
BIOCHEMISTRY
BLOOD VESSELS
BODY
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CHEMICAL REACTIONS
CHEMISTRY
CHOLESTEROL
ENZYME INDUCTION
ENZYMES
ESTERASES
ESTERIFICATION
ESTERS
GENE REGULATION
HYDROGEN COMPOUNDS
HYDROLASES
HYDROXY COMPOUNDS
LIPASES
LIPIDS
MAMMALS
MONOCARBOXYLIC ACIDS
MUSCLES
OLEIC ACID
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHOLIPIDS
PROTEINS
RATS
RODENTS
STEROIDS
STEROLS
TRITIUM COMPOUNDS
VERTEBRATES