Effects of RHC 80267, a diglyceride lipase inhibitor, on prolactin secretion and calcium uptake in GH/sub 3/ pituitary cells

Journal Article · Mon Jan 19 04:00:00 EST 1987 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(87)90343-2· OSTI ID:6803065

Camoratto, A M; Grandison, L

The effect of the diglyceride lipase inhibitor RHC 80267 on the prolactin secretory process was examined in clonal anterior pituitary GH/sub 3/ cells. This compound reduced basal prolactin secretion as well as secretion induced by TRH and phospholipase C but not that induced by phorbol myristate acetate. Although exogenous phospholipase C increased diglyceride, no increase in the products of diglyceride lipase was detected. Moreover, low doses of RHC 80267 were observed to effectively block potassium-stimulated /sup 45/calcium influx. It is unlikely that RHC 80267 inhibits prolactin release solely by inhibiting diglyceride lipase. These data suggest blockage of plasma membrane calcium channels as an alternate mechanism for the inhibitory actions of RHC 80267 on intact GH/sub 3/ cells. These observations may have implications for RHC 80267 action in other cell types.

• Research Organization: UMDNJ-Rutgers Medical School, Piscataway
• OSTI ID: 6803065
• Journal Information: Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 40:3; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METALS
ANIMAL CELLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BODY
CALCIUM
CARBOXYLESTERASES
ELEMENTS
ENDOCRINE GLANDS
ENZYME INHIBITORS
ENZYMES
ESTERASES
GLANDS
GONADOTROPINS
HORMONES
HYDROLASES
KINETICS
LIPASE
LTH
MAMMALS
METALS
ORGANS
PEPTIDE HORMONES
PITUITARY GLAND
PITUITARY HORMONES
RATS
REACTION KINETICS
RODENTS
UPTAKE
VERTEBRATES