Angiotensin II stimulates phospholipases C and A/sub 2/ in cultured rat mesangial cells
Angiotensin II stimulates prostaglandin (PG) E/sub 2/ formation in mesangial cells cultured from rat renal glomeruli. The interactions between angiotensin II and PGE/sub 2/ are important in modulating glomerular function. The authors examined the mechanism for stimulation of PGE/sub 2/ production in mesangial cells using the putative diacylglycerol-lipase inhibitor RHC 80267 and trifluoperazine (TFP), an agent interfering with Ca/sup 2 +/-CaM-mediated processes. Although RHC 80267 inhibited diacylglycerol-lipase activity in mesangial cells, it did not influence PGE/sub 2/ production in response to either angiotensin II or A23187. TFP also decreased /sup 14/C release in response to either angiotensin II of A23187. In contrast, TFP (50 ..mu..M) inhibited basal PGE/sub 2/ production and stimulation by angiotensin II and A23187. TFP also decreased /sup 14/C release in response to angiotensin from cells prelabeled with (/sup 14/C)arachidonic acid, which was associated with inhibition of /sup 14/C loss from phosphatidylinositol. In cells prelabeled with /sup 32/P, orthophosphate angiotensin II caused a rapid hydrolysis of phosphatidylinositol 4,5-bisphosphate. TFP enhanced formation of (/sup 3/H)inositol trisphosphate both under basal- and angiotensin II-stimulated conditions. Thus TFP did not inhibit phospholipase C activation by angiotensin. Angiotensin II caused marked increases in (/sup 32/P)lysophospholipids, indicating activation of also phospholipase A/sub 2/. Taken together, these results are consistent with stimulation of both phospholipase C and A/sub 2/ by angiotensin, the latter step responsible for the release of arachidonic acid and PGE/sub 2/ formation.
- Research Organization:
- Albert Einstein College of Medicine, Bronx, NY (USA)
- OSTI ID:
- 6143089
- Journal Information:
- Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 253:1; ISSN AJPHA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ANGIOTENSIN
ANIMALS
ARACHIDONIC ACID
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLESTERASES
CARBOXYLIC ACIDS
CARDIOVASCULAR AGENTS
CELL CULTURES
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DRUGS
ENZYME INDUCTION
ENZYMES
ESTERASES
ESTERS
GENE REGULATION
GLOBULINS
HYDROLASES
IMMUNOASSAY
IMMUNOLOGY
INOSITOL
INOSITOLS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPASE
LIPIDS
MAMMALS
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROSTAGLANDINS
PROTEINS
RADIOASSAY
RADIOCHROMATOGRAPHY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
RATS
RODENTS
SACCHARIDES
SEPARATION PROCESSES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VASOCONSTRICTORS
VERTEBRATES