Intestinal mucosa in diabetes: synthesis of total proteins and sucrase-isomaltase

Journal Article · Sun Jun 01 04:00:00 EDT 1986 · Am. J. Physiol.; (United States)

OSTI ID:7118502

Olsen, W A; Perchellet, E; Malinowski, R L

The effects of insulin deficiency on nitrogen metabolism in muscle and liver have been extensively studied with recent in vivo demonstration of impaired protein synthesis in rats with streptozotocin-induced diabetes. Despite the significant contribution of small intestinal mucosa to overall protein metabolism, the effect of insulin deficiency on intestinal protein synthesis have not been completely defined. The authors studied the effects of streptozotocin-induced diabetes on total protein synthesis by small intestinal mucosa and on synthesis of a single enzyme protein of the enterocyte brush-border membrane sucrase-isomaltase. They used the flood-dose technique to minimize the difficulties of measuring specific radioactivity of precursor phenylalanine and determined incorporation into mucosal proteins and sucrase-isomaltase 20 min after injection of the labeled amino acid. Diabetes did not alter mucosal mass as determined by weight and content of protein and DNA during the 5 days after injection of streptozotocin. Increased rates of sucrase-isomaltase synthesis developed beginning on day 3, and those of total protein developed on day 5. Thus intestinal mucosal protein synthesis is not an insulin-sensitive process.

• Research Organization: Univ. of Wisconsin, Madison
• OSTI ID: 7118502
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 250:6; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
BIOSYNTHESIS
BODY
CARBOXYLIC ACIDS
DIABETES MELLITUS
DIGESTIVE SYSTEM
DISEASES
ENDOCRINE DISEASES
ENZYMES
GASTROINTESTINAL TRACT
GLYCOSYL HYDROLASES
HYDROLASES
INTESTINES
LABELLED COMPOUNDS
MAMMALS
MEMBRANES
METABOLIC DISEASES
MUCOUS MEMBRANES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PHENYLALANINE
PHYSIOLOGY
PROTEINS
RATS
RODENTS
SMALL INTESTINE
SYNTHESIS
TRITIUM COMPOUNDS
VERTEBRATES