Nature of elevated rat intestinal carbohydrase activities after high-carbohydrate diet feeding

Journal Article · Tue Oct 01 04:00:00 EDT 1985 · Am. J. Physiol.; (United States)

OSTI ID:6287618

Tsuboi, K K; Kwong, L K; Yamada, K; Sunshine, P; Koldovsky, O

Adult rats that were maintained on a low-carbohydrate intake showed rapid increase in the activities of sucrase, maltase, and lactase along the length of the small intestine when they were fed a high-starch diet. In the present study, the authors have identified these activity increases, and showed that they reflect proportional accumulations in enzyme-protein of sucrase-isomaltase, maltase-glucoamylase, and neutral lactase. It was determined that each of these enzymes exists in adult rat intestine in single immunoreactive form and accounts as a group for all sucrase, cellobiase, and most maltase and lactase activities. Dietary change from low to high carbohydrate (starch) resulted in an increase in (TH)leucine accumulation in each of the enzymes, without a change in the amount of label accumulation in total intestinal proteins. The increase in label accumulation in the brush-border carbohydrase pools was matched generally by proportional elevation in the pool concentrations of sucrase-isomaltase and lactase but not maltase. These studies suggest that the elevation of intestinal carbohydrase concentrations induced by high-carbohydrate feeding may involve selective stimulation of their synthesis.

• Research Organization: Stanford Univ. School of Medicine, CA
• OSTI ID: 6287618
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States); ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
DIET
DIGESTIVE SYSTEM
ENZYME ACTIVITY
ENZYMES
GASTROINTESTINAL TRACT
GLYCOSYL HYDROLASES
HYDROLASES
INTESTINES
LABELLED COMPOUNDS
LEUCINE
MAMMALS
O-GLYCOSYL HYDROLASES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PHYSIOLOGY
RATS
RODENTS
TRITIUM COMPOUNDS
VERTEBRATES