Protein tyrosine phosphorylation during meiotic divisions of starfish oocytes

Journal Article · Sun Apr 01 04:00:00 EDT 1990 · Developmental Biology; (USA)

DOI:https://doi.org/10.1016/0012-1606(90)90205-W· OSTI ID:7118079

Peaucellier, G; Andersen, A C; Kinsey, W H ^[1]

---

1. Univ. of Miami School of Medicine, FL (USA)

We have used an antibody specific for phosphotyrosine to investigate protein phosphorylation on tyrosine during hormone-induced maturation of starfish oocytes. Analysis of immunoprecipitates from cortices of in vivo labeled Marthasterias glacialis oocytes revealed the presence of labeled phosphotyrosine-containing proteins only after hormone addition. Six major phosphoproteins of 195, 155, 100, 85, 45, and 35 kDa were detected. Total activity in immunoprecipitates increased until first polar body emission and was greatly reduced upon completion of meiosis but some proteins exhibited different kinetics. The labeling of the 155-kDa protein reached a maximum at germinal vesicle breakdown, while the 35-kDa appeared later and disappeared after polar body emission. Similar results were obtained with Asterias rubens oocytes. In vitro phosphorylation of cortices showed that tyrosine kinase activity is a major protein kinase activity in this fraction, the main endogenous substrate being a 68-kDa protein. The proteins phosphorylated on tyrosine in vitro were almost similar in extracts from oocytes treated or not with the hormone.

• OSTI ID: 7118079
• Journal Information: Developmental Biology; (USA), Journal Name: Developmental Biology; (USA) Vol. 138:2; ISSN 0012-1606; ISSN DEBIA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
ANTIBODIES
AQUATIC ORGANISMS
CARBOXYLIC ACIDS
CELL DIVISION
CHEMICAL REACTIONS
ECHINODERMS
ENZYME ACTIVITY
ENZYMES
GAMETOGENESIS
GERM CELLS
HYDROXY ACIDS
INVERTEBRATES
ISOTOPE APPLICATIONS
ISOTOPES
MEMBRANE PROTEINS
MOLECULAR WEIGHT
OOCYTES
OOGENESIS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHOPROTEINS
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
PROTEINS
SPECIFICITY
TRACER TECHNIQUES
TRANSFERASES
TYROSINE