Protein tyrosine phosphorylation during meiotic divisions of starfish oocytes
- Univ. of Miami School of Medicine, FL (USA)
We have used an antibody specific for phosphotyrosine to investigate protein phosphorylation on tyrosine during hormone-induced maturation of starfish oocytes. Analysis of immunoprecipitates from cortices of in vivo labeled Marthasterias glacialis oocytes revealed the presence of labeled phosphotyrosine-containing proteins only after hormone addition. Six major phosphoproteins of 195, 155, 100, 85, 45, and 35 kDa were detected. Total activity in immunoprecipitates increased until first polar body emission and was greatly reduced upon completion of meiosis but some proteins exhibited different kinetics. The labeling of the 155-kDa protein reached a maximum at germinal vesicle breakdown, while the 35-kDa appeared later and disappeared after polar body emission. Similar results were obtained with Asterias rubens oocytes. In vitro phosphorylation of cortices showed that tyrosine kinase activity is a major protein kinase activity in this fraction, the main endogenous substrate being a 68-kDa protein. The proteins phosphorylated on tyrosine in vitro were almost similar in extracts from oocytes treated or not with the hormone.
- OSTI ID:
- 7118079
- Journal Information:
- Developmental Biology; (USA), Vol. 138:2; ISSN 0012-1606
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANTIBODIES
SPECIFICITY
ECHINODERMS
OOGENESIS
PHOSPHOPROTEINS
PHOSPHORYLATION
PHOSPHOTRANSFERASES
ENZYME ACTIVITY
CELL DIVISION
MEMBRANE PROTEINS
MOLECULAR WEIGHT
OOCYTES
PHOSPHATES
PHOSPHORUS ISOTOPES
TRACER TECHNIQUES
TYROSINE
AMINO ACIDS
ANIMALS
AQUATIC ORGANISMS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
ENZYMES
GAMETOGENESIS
GERM CELLS
HYDROXY ACIDS
INVERTEBRATES
ISOTOPE APPLICATIONS
ISOTOPES
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PROTEINS
TRANSFERASES
550201* - Biochemistry- Tracer Techniques