Dimethylthiourea decreases acute lung edema in phorbol myristate acetate-treated rabbits

Journal Article · Tue Jul 01 04:00:00 EDT 1986 · Am. J. Physiol.; (United States)

OSTI ID:7117963

Jackson, J H; White, C W; McMurtry, I F; Berger, E M; Repine, J E

Treatment with dimethylthiourea (DMTU), a potent O/sub 2/ metabolite scavenger, prevented neutrophil-mediated acute edema in lungs of rabbits given phorbol myristate acetate (PMA) and in isolated rabbit lungs perfused with neutrophils and PMA. DMTU-treated rabbits given PMA did not increase their lung weight-to-total body weight ratios (5.0 +/- 0.3) or lung lavage albumin concentrations (14 +/- 4.6 mg/dl) in comparison to untreated rabbits given PMA (6.6 +/- 0.5 and 60 +/- 10 mg/dl, respectively). Similarly, DMTU-treated isolated rabbit lungs perfused with neutrophils and PMA did not gain weight (0 g) or increase their lavage albumin concentrations (82 +/- 17 mg/dl) in comparison to untreated lungs perfused with neutrophils and PMA (71 +/- 3.1 g and 1299 +/- 47 mg/dl, respectively). DMTU did not appear to decrease edema by preventing increases in pulmonary arterial pressures (PAP). First, treatment with DMTU did not decrease initial PAP increases in rabbits given PMA. Second, even though addition of DMTU attenuated PAP increased in isolated lungs perfused with neutrophils and PMA, DMTU-treated isolated lungs did not develop acute edema when subjected to mechanical increases in venous outflow pressures. The mechanism by which DMTU decreases lung edema is unclear by may involve scavenging of toxic O/sub 2/ metabolites, since DMTU also decrease hydrogen peroxide (H/sub 2/O/sub 2/) and hydroxyl radical (OH) concentrations in in vitro mixtures containing neutrophils and PMA.

• Research Organization: Univ. of Colorado, Denver
• OSTI ID: 7117963
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 61:1; ISSN AJPHA
• Country of Publication: United States
• Language: English

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560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETATES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBONIC ACID DERIVATIVES
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
CARCINOGENS
EDEMA
ESTERS
LEUKOCYTES
LUNGS
MATERIALS
MONOCARBOXYLIC ACIDS
NEUTROPHILS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PHORBOL ESTERS
PHYSIOLOGY
RESPIRATORY SYSTEM
SYMPTOMS
TETRADECANOIC ACID
THIOUREAS