Evidence suggesting a role for hydroxyl radical in passive Heymann nephritis in rats

Journal Article · Tue Mar 01 04:00:00 EST 1988 · American Journal of Physiology; (USA)

OSTI ID:6867849

Shah, S V ^[1]

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1. Tulane Medical School and Veterans Administration Hospital, New Orleans, LA (USA)

The authors examined the effect of scavengers of reactive oxygen metabolites on proteinuria in the passive Heymann nephritis model of membranous nephropathy. Passive Heymann nephritis was induced by a single intravenous injection of anti-Fx1A IgG in a dose of 10 mg/100 g body weight. Superoxide dismutase, a scavenger of superoxide or catalase which destroys hydrogen peroxide, did not affect the proteinuria. In contrasts, dimethylthiourea (DMTU), a scavenger of hydroxyl radical, markedly reduced the proteinuria. Experiments with {sup 125}I-labeled anti-Fx1A antibody demonstrated that DMTU did not affect the amount of antibody deposited in the kidney. Semiquantitative estimation of IgG and complement deposition in the kidneys showed no differences between the DMTU-treated and control rats. A second hydroxyl radical scavenger, sodium benzoate also resulted in marked reduction in proteinuria. Because of the participation of iron in biological systems to generate hydroxyl radical, they also examined the effect of deferoxamine (DFO) an iron chelator, on the anti-Fx1A-induced proteinuria. There was a significant reduction in proteinuria in rats treated concurrently with DFO. These results suggest a potential role of the hydroxyl radical in passive Heymann nephritis.

• OSTI ID: 6867849
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 254:3; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMALS
AROMATICS
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CARBONIC ACID DERIVATIVES
CARBOXYLIC ACIDS
CHELATING AGENTS
DAYS LIVING RADIOISOTOPES
DEFEROXAMINE
DISEASES
DYES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
FLUORESCEIN
GLOBULINS
HYDROXY ACIDS
HYDROXY COMPOUNDS
HYDROXYL RADICALS
IMMUNOGLOBULINS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NEPHRITIS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXIDOREDUCTASES
PATHOGENESIS
PHENOLS
POLYPHENOLS
PROTEINS
RADICALS
RADIOISOTOPES
RATS
RODENTS
SUPEROXIDE DISMUTASE
THIOUREAS
TRACER TECHNIQUES
UROGENITAL SYSTEM DISEASES
VERTEBRATES