Adenosine (ADO) prevents phorbol myristate acetate (PMA) injury in rabbit lungs: Role of leukotrienes (LT)

Conference · Mon Mar 11 04:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5300123

Zanaboni, P; Bradley, J; Webster, R; Baudendistel, L; Dahms, T ^[1]

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1. St. Louis Univ., MO (United States)

Pretreatment with ADO prevents the PMA-induced increase in the fluid filtration coefficient (K{sub f}) in isolated perfused rabbit lungs. ADO can block the synthesis of LTs. This study determined whether the protective effect of ADO in PMA induced injury was due to decreased LT production. Isolated rabbit lungs were perfused with a 1:1 mixture of blood and 6% albumin in Krebs-Henseleit buffer. K{sub f} was measured after hemodynamic and weight stabilization and then 30 min after PMA. Perfusate samples were collected after each K{sub f} measurement for determination of LTC{sub 4} and LTD{sub 4} by RIA. The specific role of LT was studied by pretreating with MK-886, a LT synthesis inhibitor. In the vehicle pretreatment group, PMA caused a significant increase in K{sub f} and LTC{sub 4} + LTD{sub 4}. ADO inhibited both the increase in K{sub f} and in LTC{sub 4} + LTD{sub 4}. Pretreatment with MK-886 blocked the PMA-induced increase in LT but did not inhibit the K{sub f} increase. ADO alters the production of LTC{sub 4} + LTD{sub 4} following PMA; but it is probably not the mechanism by which ADO prevents the increase in microvascular permeability.

• OSTI ID: 5300123
• Report Number(s): CONF-9104107--
• Conference Information: Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 5:4
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADENOSINE
ANIMALS
BIOASSAY
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BIOSYNTHESIS
BODY
CARCINOGENS
DIAGNOSTIC TECHNIQUES
ESTERS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LUNGS
MAMMALS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
PERFUSED ORGANS
PHORBOL ESTERS
PROSTAGLANDINS
RABBITS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RESPIRATORY SYSTEM
RIBOSIDES
SYNTHESIS
TOXICITY
TRACER TECHNIQUES
VERTEBRATES