Effect of verapamil and OKY-046 on phorbol myristate acetate (PMA)-induced lung injury

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5499188

Allison, R C; Marble, K T; Hernandez, E M; Townsley, M I; Taylor, A E

In isolated dog lungs perfused at constant pressure, PMA (25-50 ..mu..g) was injected into the recirculating perfusate (vol = 550 ml), and its effect on vascular permeability, as determined by filtration coefficient (Kf) and isogravimetric capillary pressure (Pci), and on pulmonary vascular resistance (PVR) was observed. Group 1 lungs (n = 5) were perfused with plasma/dextran solution; Group 2 lungs (n = 6) were perfused with blood; Group 3 (n = 5) were blood-perfused and pretreated with 5 mg verapamil; and Group 4 (n = 3) were blood-perfused and pretreated with 100 mg OKY-046. Baseline (BL) values for Kf (g/min/cmH/sub 2/O/100g lung), Pci (cmH/sub 2/O), and PVR (cmH/sub 2/O/L/min/100g lung) are compared to those obtained one hour (1 Hr) after PMA (p < 0.05). The authors conclude that cellular components of blood are required for the permeability injury but not the increase in PVR, and the increase in permeability can be attenuated by a calcium-channel blocker or a thromboxane synthetase inhibitor.

• Research Organization: Univ. of South Alabama, Mobile
• OSTI ID: 5499188
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism &amp; Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BLOOD VESSELS
BODY
CARCINOGENS
CARDIOVASCULAR AGENTS
CARDIOVASCULAR SYSTEM
DOGS
DRUGS
ESTERS
INJURIES
LUNGS
MAMMALS
ORGANIC COMPOUNDS
ORGANS
PERFUSED ORGANS
PERMEABILITY
PHORBOL ESTERS
RESPIRATORY SYSTEM
TOXICITY
VASODILATORS
VERTEBRATES