Radioimmunoassay of chlordiazepoxide in plasma

Journal Article · Sun Jun 01 04:00:00 EDT 1975 · J. Pharm. Sci.; (United States)

DOI:https://doi.org/10.1002/jps.2600640607· OSTI ID:7112602

Dixon, W R; Earley, J; Postma, E

A simple, rapid, and sensitive radioimmunoassay was developed for the determination of chlordiazepoxide (7-chloro-2-methylamino-5-phenyl-3H-1,4-benzodiazepine 4-oxide) in plasma. Antiserum capable of binding chlordiazepoxide-2-/sup 14/C was obtained from rabbits following immunization with an antigen prepared by coupling the reactive acyl azide of 7-chloro-5-(4-hydrazinocarbonylmethoxyphenyl)-2-methylamino-3H-1,4-benzodiazepine 4-oxide to bovine serum albumin. The radioimmunoassay allows for the specific determination of chlordiazepoxide directly in plasma without extraction and was compared with a differential spectrofluorometric assay for chlordiazepoxide. Both methods gave satisfactory agreement for the plasma levels of chlordiazepoxide in human subjects resulting from single and chronic oral doses of chlordiazepoxide hydrochloride.

• OSTI ID: 7112602
• Journal Information: J. Pharm. Sci.; (United States), Journal Name: J. Pharm. Sci.; (United States) Vol. 64:6; ISSN JPMSA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIGENS
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY FLUIDS
CARBON 14 COMPOUNDS
DRUGS
IMMUNE SERUMS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
PATIENTS
PSYCHOTROPIC DRUGS
RADIOASSAY
RADIOIMMUNOASSAY
TRACER TECHNIQUES