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Solanum glaucophyllum as source of 1,25-dihydroxyvitamin D/sub 3/

Journal Article · · J. Biol. Chem.; (United States)
OSTI ID:7110271
; ; ; ;

Vitamin D-deficient rats given an aqueous extract of the South American plant Solanum glaucophyllum accumulate 1,25-dihydroxyvitamin D/sub 3/ in their blood and intestines at the time they show enhanced intestinal calcium absorption. The identity of the 1,25-dihydroxyvitamin D/sub 3/ was established by co-chromatography with 1,25-dihydroxy(23,24-/sup 3/H)vitamin D/sub 3/ on Sephadex LH-20 columns, microparticulate silica gel columns, a reversed-phase column developed under high pressure, and by a specific 1,25-dihydroxyvitamin D/sub 3/ binding assay. The chromatographic systems used are fully capable of resolving all of the known metabolites of vitamin D/sub 3/. Serum of the S. glaucophyllum-treated rats showed 300 pg/ml of 1,25-dihydroxyvitamin D/sub 3/ and no detectable 1,25-dihydroxyvitamin D/sub 2/. Similarly, intestine of such rats had 230 pg/g of 1,25-dihydroxyvitamin D/sub 3/. Control animals which received the vehicle instead of S. glaucophyllum had only 20 pg/ml of 1,25-dihydroxyvitamin D/sub 3/ in their serum and 4.4 pg/g of 1,25-dihydroxyvitamin D/sub 2/ in their intestine. These results demonstrate that S. glaucophyllum extracts must be a source of 1,25-dihydroxyvitamin D/sub 3/; thus a significant basis for the calcinogenic properties of S. glaucophyllum must be the presence of a conjugated form of 1,25-dihydroxyvitamin D/sub 3/, which is rendered available by digestion.

Research Organization:
Univ. of Wisconsin, Madison
OSTI ID:
7110271
Journal Information:
J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 252:8; ISSN JBCHA
Country of Publication:
United States
Language:
English

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Related Subjects

550500* -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
ADSORBENTS
ALKALINE EARTH METALS
ANIMALS
BIOLOGICAL MATERIALS
BIOMASS
BLOOD
BODY
BODY FLUIDS
CALCIUM
CHOLECALCIFEROL
CHROMATOGRAPHY
DIGESTIVE SYSTEM
ELEMENTS
ENERGY SOURCES
GASTROINTESTINAL TRACT
INTESTINAL ABSORPTION
INTESTINES
MAMMALS
METABOLISM
METALS
NUTRITIONAL DEFICIENCY
ORGANS
PLANTS
RATS
RENEWABLE ENERGY SOURCES
RODENTS
SEPARATION PROCESSES
SILICA GEL
UPTAKE
VERTEBRATES
VITAMIN D
VITAMINS