Localization of 1,25-dihydroxyvitamin D/sub 3/ in intestinal nuclei in vivo

Journal Article · Tue Jan 31 23:00:00 EST 1978 · Arch. Biochem. Biophys.; (United States)

DOI:https://doi.org/10.1016/0003-9861(78)90458-7· OSTI ID:5637013

Zile, M; Bunge, E C; Barsness, L; Yamada, S; Schnoes, H K; DeLuca, H F

Autoradiography of frozen sections of intestinal tissue taken from rachitic chickens given a single intravenous dose of 1,25-dihydroxy(23,24/sup 3/H)vitamin D/sub 3/ (650 pmol, 78 Ci/mmol) has been carried out. Specific localization of label in the nuclei of intestinal villi and crypt cells could be demonstrated at 2.5 to 6 h postintection. In contrast, no concentration or localization of radioactivity could be detected in intestinal muscle, liver, and skeletal muscle. These results strongly support the concept that the function of 1,25-dihydroxyvitamin D/sub 3/ in intestine is mediated by a nuclear mechanism.

• Research Organization: Univ. of Wisconsin, Madison
• OSTI ID: 5637013
• Journal Information: Arch. Biochem. Biophys.; (United States), Journal Name: Arch. Biochem. Biophys.; (United States) Vol. 186:1; ISSN ABBIA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METALS
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL PATHWAYS
BIRDS
BODY
CALCIUM
CELL CONSTITUENTS
CELL NUCLEI
CHICKENS
CHOLECALCIFEROL
DIGESTIVE SYSTEM
ELEMENTS
EPITHELIUM
FOWL
GASTROINTESTINAL TRACT
HYDROGEN ISOTOPES
INTESTINAL ABSORPTION
INTESTINES
ISOTOPES
LIGHT NUCLEI
METALS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
RADIOISOTOPES
TISSUES
TRITIUM
UPTAKE
VERTEBRATES
VITAMIN D
VITAMINS
YEARS LIVING RADIOISOTOPES