Organ distribution of gut-derived bacteria caused by bowel manipulation or ischemia

Journal Article · Mon Jan 01 04:00:00 EST 1990 · American Journal of Surgery; (USA)

DOI:https://doi.org/10.1016/S0002-9610(05)80611-7· OSTI ID:7105265

Redan, J A; Rush, Jr, B F; Lysz, T W; Smith, S; Machiedo, G W ^[1]

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1. Univ. of Medicine and Dentistry of New Jersey, Newark (USA)

Translocation of carbon-14-labeled Escherichia coli from the gut was studied at the specified times in the following groups of rats: Group 1, 5 hours after ligation of the superior mesenteric artery; Group 2, 5 hours after laparotomy and exposure of the superior mesenteric artery with gentle removal and replacement of the intestines; and Group 3, 5 hours after handling but no surgical manipulation. Both living and dead bacteria were administered by means of gavage, and the effect of viability, intestinal ischemia without reperfusion, and bowel manipulation on the translocation of enteric bacteria was assessed. We demonstrated that (1) even gentle bowel manipulation causes bacteremia as great as that associated with ligation of the superior mesenteric artery; (2) dead E. coli are absorbed into the blood in the presence of bowel manipulation or ischemia but less effectively than are live E. coli; (3) live bacteria are found in highest concentration in the lung and in descending order in the liver, kidney, heart, and spleen; (4) dead bacteria absorbed from the gut are found in highest concentration in the kidney and the liver. Lesser amounts are found in the lung, spleen, and heart.

• OSTI ID: 7105265
• Journal Information: American Journal of Surgery; (USA), Journal Name: American Journal of Surgery; (USA) Vol. 159:1; ISSN 0002-9610; ISSN AJSUA
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ARTERIES
BACTERIA
BACTERIAL DISEASES
BIOLOGICAL MATERIALS
BLOOD
BLOOD VESSELS
BODY
BODY FLUIDS
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
DIGESTIVE SYSTEM
DISEASES
DISTRIBUTION
ESCHERICHIA COLI
GASTROINTESTINAL TRACT
GLANDS
HEART
INFECTIOUS DISEASES
INTESTINES
ISCHEMIA
KIDNEYS
LIVER
LUNGS
MAMMALS
MATERIALS
MEDICINE
MICROORGANISMS
ORAL ADMINISTRATION
ORGANS
PATHOGENESIS
RATS
RESPIRATORY SYSTEM
RODENTS
SPLEEN
SURGERY
TISSUE DISTRIBUTION
VASCULAR DISEASES
VERTEBRATES
VIABILITY