Effect of biliary obstruction on a gram-negative bacteremic challenge: a preliminary report

Journal Article · Sun Jun 01 04:00:00 EDT 1986 · Surgery; (United States)

OSTI ID:5330618

Scott-Conner, C E; Bernstein, J M; Scher, K S; Mack, M E

Alterations in the sequestration and destruction of bacteria were studied after 10 days of biliary obstruction. Intraperitoneal injection of radiolabeled Escherichia coli was used to study bacterial localization in rats 10 days after common duct ligation and transection or sham celiotomy. Animals were sacrificed 4 hours later and uptake by liver, spleen, lung, and kidney were studied with a scintillation counter. No significant difference in localization between the two groups was noted. Bacteremia was induced in a second set of animals and quantitative bacterial organ cultures were performed. Significantly more viable organisms were identified in lung, liver, and kidney of animals that underwent common duct ligation and transection, when compared with controls that underwent sham celiotomy. This suggests that there is a defect in bacterial killing after 10 days of biliary obstruction. The inability to effectively clear and kill gram-negative bacteria in patients with biliary obstruction may account for some of the clinical complications seen in this patient population.

• Research Organization: Huntington Veterans Administration Medical Center, VA
• OSTI ID: 5330618
• Journal Information: Surgery; (United States), Journal Name: Surgery; (United States) Vol. 6; ISSN SURGA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
BACTERIA
BILIARY TRACT
BODY
COUNTING TECHNIQUES
DIGESTIVE SYSTEM
DIGESTIVE SYSTEM DISEASES
DISEASES
ESCHERICHIA COLI
GLANDS
ISOTOPE APPLICATIONS
LIVER
MAMMALS
MICROORGANISMS
ORGANS
PATHOGENESIS
RATS
RODENTS
SCINTILLATION COUNTING
SEPTICEMIA
SPLEEN
TRACER TECHNIQUES
VERTEBRATES
VIABILITY