Intestinal permeability and bacterial translocation following small bowel transplantation in the rat

Journal Article · Thu Aug 01 04:00:00 EDT 1991 · Transplantation; (United States)

DOI:https://doi.org/10.1097/00007890-199108000-00007· OSTI ID:5227786

Grant, D; Hurlbut, D; Zhong, R; Wang, P Z; Chen, H F; Garcia, B; Behme, R; Stiller, C; Duff, J ^[1]

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1. University of Western Ontario (Canada)

In addition to its role in absorbing nutrients, the intestinal mucosa provides an important barrier against toxins and bacteria in the bowel lumen. The present study evaluated gut barrier function following orthotopic (in continuity) intestinal grafting in rats. Graft histology, intestinal permeability, and bacterial translocation to the grafted mesenteric lymph nodes, the host's liver, and the host's spleen were assessed on the 3rd, 5th, and 7th postoperative days. The study group received no immunosuppression after allotransplantation. The two control groups included rats with isografts and rats with cyclosporine-treated allografts. On the 7th POD, the study animals had moderate transmural inflammation due to rejection, with normal histology in the isografts and CsA-treated allografts; increased intestinal permeability, measured by urinary excretion of oral 51Cr-EDTA (P less than 0.01); and increased number of bacteria in the MLN and spleen (P less than 0.05). The number of bacteria in the MLN and spleen of the study group positively correlated with the changes in intestinal permeability (P less than 0.05). Rejection of the orthotopic intestinal graft leads to increased intestinal permeability and bacterial translocation from the lumen of the graft to the host's reticuloendothelial system. Measures to improve gut barrier function and antibiotic therapy during rejection episodes may help reduce the incidence of septic complications after intestinal grafting.

• OSTI ID: 5227786
• Journal Information: Transplantation; (United States), Journal Name: Transplantation; (United States) Vol. 52:2; ISSN 0041-1337; ISSN TRPLA
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION
AMINO ACIDS
ANIMAL TISSUES
ANIMALS
BACTERIAL DISEASES
BETA DECAY RADIOISOTOPES
BIOLOGICAL FUNCTIONS
BODY
CARBOXYLIC ACIDS
CHELATING AGENTS
CHROMIUM 51
CHROMIUM ISOTOPES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
EDTA
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GASTROINTESTINAL TRACT
GLANDS
GRAFT-HOST REACTION
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
INTESTINAL ABSORPTION
INTESTINES
ISOTOPES
LIVER
LYMPH NODES
LYMPHATIC SYSTEM
MAMMALS
MEMBRANES
MESENTERY
MUCOUS MEMBRANES
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PERMEABILITY
RADIOISOTOPES
RATS
RETICULOENDOTHELIAL SYSTEM
RODENTS
SEROUS MEMBRANES
SMALL INTESTINE
SPLEEN
TISSUES
TRANSPLANTS
UPTAKE
VERTEBRATES