Nucleotide and deduced amino acid sequence of a human cDNA (NQO sub 2 ) corresponding to a second member of the NAD(P)H:quinone oxidoreductase gene family. Extensive polymorphism at the NQO sub 2 gene locus on chromosome 6

Journal Article · Tue Feb 20 04:00:00 EST 1990 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00459a034· OSTI ID:7101129

Jaiswal, A K; Adesnik, M ^[1]; Burnett, P; McBride, O W ^[2]

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1. New York Univ. Medical Center, NY (USA)
2. National Institutes of Health, Bethesda, MD (USA)

NAD(P)H:quinone oxidoreductases (NQOs) are flavoproteins that catalyze the oxidation of NADH or NADPH by various quinones and oxidation-reduction dyes. The authors previously described a complementary DNA that encodes a dioxin-inducible cytosolic form of human NAD(P)H:quinone oxidoreductase (NQO{sub 1}). In the present report they describe the nucleotide sequence and deduced amino acid sequence for a cDNA clone that is likely to encode a second form of NAD(P)H:quinone oxidoreductase (NQO{sub 2}) which was isolated by screening a human liver cDNA library by hybridization with a NQO{sub 1} cDNA probe. The NQO{sub 2} cDNA is 976 nucleotides long and encodes a protein of 231 amino acids. COS1 cells transfected with NQO{sub 2} cDNA showed a 5-7 fold increase in NAD(P)H:quinone oxidoreductase activity as compared to nontransfected cells when either 2,6-dichlorophenolindophenol or menadione was used as substrate. The NQO{sub 2} gene locus is highly polymorphic as indicated by several restriction fragment length polymorphisms detected with five different restriction enzymes. The NQO{sub 2} gene was localized to human chromosome 6 by Southern analysis of human-rodent somatic cell hybrids.

• OSTI ID: 7101129
• Journal Information: Biochemistry; (USA), Journal Name: Biochemistry; (USA) Vol. 29:7; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMALS
AROMATICS
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
DNA
DNA HYBRIDIZATION
DNA SEQUENCING
ENZYMES
GENETIC MAPPING
HETEROCHROMOSOMES
HYBRIDIZATION
MAMMALS
MAN
MAPPING
MOLECULAR STRUCTURE
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
OXIDOREDUCTASES
PRIMATES
QUINONES
RECOMBINANT DNA
RFLPS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES