cDNA sequence, protein structure, and chromosomal location of the human gene for poly(ADP-ribose) polymerase
Recently the authors described a full-length cDNA for the human nuclear enzyme poly(ADP-ribose) polymerase. Here, they report the chromosomal localization and partial map of the human gene for this enzyme as well as the complete coding sequence for this protein. The nucleotide sequence reveals a single 3042-base open reading frame encoding a protein with a predicted M/sub r/ of 113,135. A comparison of this deduced amino acid sequence with the amino acid sequence of three peptides derived from human poly(ADP-ribose) polymerase revealed a match of 27 amino acid residues. A computer-derived structural analysis of the enzyme and a search for similarities with other proteins confirmed that the polymerase belongs to a subfamily of DNA/NAD-binding proteins and DNA-repair proteins. Possible Zn/sup 2 +/-binding fingers, a nucleotide-binding fold, and a nuclear transport signal were noted. Additionally, chromosomal mapping has identified polymerase-hybridizing sequences on human chromosomes 1 (the active gene), 13, and 14 (processed pseudogenes). Using the polymerase cDNA as a probe, they also have detected several DNA restriction fragment length polymorphisms in normal humans.
- Research Organization:
- Georgetown Univ. Schools of Medicine and Dentistry, Washington, DC (USA)
- OSTI ID:
- 6468240
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:23
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GENES
DNA SEQUENCING
GENETIC MAPPING
POLYMERASES
AMINO ACID SEQUENCE
CATIONS
HETEROCHROMOSOMES
HYBRIDIZATION
MAN
PHOSPHORUS 32
RFLPS
ZINC COMPOUNDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHARGED PARTICLES
CHROMOSOMES
DAYS LIVING RADIOISOTOPES
ENZYMES
IONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAPPING
MOLECULAR STRUCTURE
NUCLEI
NUCLEOTIDYLTRANSFERASES
ODD-ODD NUCLEI
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PRIMATES
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
VERTEBRATES
550401* - Genetics- Tracer Techniques