cDNA sequence, protein structure, and chromosomal location of the human gene for poly(ADP-ribose) polymerase

Journal Article · Tue Dec 01 04:00:00 EST 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.23.8370· OSTI ID:6468240

Cherney, B W; McBride, O W; Chen, D; Alkhatib, H; Bhatia, K; Hensley, P; Smulson, M E

Recently the authors described a full-length cDNA for the human nuclear enzyme poly(ADP-ribose) polymerase. Here, they report the chromosomal localization and partial map of the human gene for this enzyme as well as the complete coding sequence for this protein. The nucleotide sequence reveals a single 3042-base open reading frame encoding a protein with a predicted M/sub r/ of 113,135. A comparison of this deduced amino acid sequence with the amino acid sequence of three peptides derived from human poly(ADP-ribose) polymerase revealed a match of 27 amino acid residues. A computer-derived structural analysis of the enzyme and a search for similarities with other proteins confirmed that the polymerase belongs to a subfamily of DNA/NAD-binding proteins and DNA-repair proteins. Possible Zn/sup 2 +/-binding fingers, a nucleotide-binding fold, and a nuclear transport signal were noted. Additionally, chromosomal mapping has identified polymerase-hybridizing sequences on human chromosomes 1 (the active gene), 13, and 14 (processed pseudogenes). Using the polymerase cDNA as a probe, they also have detected several DNA restriction fragment length polymorphisms in normal humans.

• Research Organization: Georgetown Univ. Schools of Medicine and Dentistry, Washington, DC (USA)
• OSTI ID: 6468240
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:23; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CATIONS
CHARGED PARTICLES
CHROMOSOMES
DAYS LIVING RADIOISOTOPES
DNA SEQUENCING
ENZYMES
GENES
GENETIC MAPPING
HETEROCHROMOSOMES
HYBRIDIZATION
IONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAN
MAPPING
MOLECULAR STRUCTURE
NUCLEI
NUCLEOTIDYLTRANSFERASES
ODD-ODD NUCLEI
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PRIMATES
RADIOISOTOPES
RFLPS
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
VERTEBRATES
ZINC COMPOUNDS