Covalently closed circular DNAs of murine type C retrovirus: depressed formation in cells treated with cycloheximide early after infection

Journal Article · Wed Oct 01 04:00:00 EDT 1980 · J. Virol.; (United States)

OSTI ID:7096566

Yang, W K; Yang, D M; Kiggans, Jr, J O

Formation of viral closed circular supercoiled DNA duplexes and production of progeny virus were both inhibited in cultured mouse cells treated with cycloheximide in the first 4 h of type C retrovirus infection. With different doses of cycloheximide to cause different degrees of inhibition, the number of viral supercoiled DNA duplexes detected in the cells at 11 h showed an apparent correlation with the amount of progeny virus produced in the 12- to 22-h period of infection. These kinetic data suggest that a cycloheximide-sensitive metabolic process, probably early viral protein synthesis, is required for retrovirus replication and supercoiled viral DNA formation in the cell.

• Research Organization: Oak Ridge National Lab., TN
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 7096566
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 36:1; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
560302* -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIBIOTICS
BIOLOGICAL EFFECTS
CONFIGURATION
CYCLOHEXIMIDE
DNA
DRUGS
FUNGICIDES
HELICAL CONFIGURATION
INHIBITION
MAMMALS
MICE
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PESTICIDES
RODENTS
SIDE EFFECTS
VERTEBRATES
VIRUSES