Restricted infectivity of ecotropic type C retroviruses in mouse teratocarcinoma cells: studies on viral DNA intermediates

Conference · Tue Jan 01 04:00:00 EST 1980

OSTI ID:5375306

Yang, W K; d'Auriol, L; Yang, D M; Kiggans, Jr, J O; Ou, C Y; Peries, J; Emanoil-Ravicovitch, R

Infectivity of retroviruses in cultured murine teratocarcinoma cells was found to be affected by the state of cellular differentiation. Present studies utilize two kinds of cell cultures from teratocarcinomas of mouse strain 129, an undifferentiated pluriopotential cell line (PCC/sub 4/) and a myoblast-derived cell line (PCD/sub 1/) which are respectively resistant and susceptible to the infection of Gross strain N-tropic type C retrovirus. Analyses of the appearance of free viral DNA intermediates in these cells from 4 to 78 h after virus inoculation were made. In both PCD/sub 1/ and PCC/sub 4/ cells, virus inoculation induced the formation of one linear form (III) and two covalently-closed supercoiled circular forms (I) of viral DNA duplexes; the linear form showing its appearance, increase, and decline in the 4 to 18 h period, and the circular forms in the 8 to 24 h period. In the period of 56 to 78 h after virus inoculation, a secondary burst of viral DNA synthesis occurred in PCD/sub 1/ cells, whereas both linear and supercoiled viral DNA duplexes became undetectable in PCC/sub 4/ cells. Free and unintegrated viral DNA preparations from PCD/sub 1/ and PCC/sub 4/ cells 10 h after virus inoculation were both infectious for N3T3 cells in a DNA transfection assay. Both PCD/sub 1/ and PCC/sub 4/ cells were very poor recipients for DNA transfection. These results indicate that restriction of retrovirus in undifferentiated teratocarcinoma cells occurs at a step beyond formation and maturation of viral DNA intermediates. (ERB)

• Research Organization: Oak Ridge National Lab., TN (USA); Hopital Saint-Louis, 75 - Paris (France)
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 5375306
• Report Number(s): CONF-800239-1
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550300 -- Cytology
550700 -- Microbiology
550900 -- Pathology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
CELL CULTURES
CELL DIFFERENTIATION
DNA REPLICATION
INFECTIVITY
MAMMALS
MICE
MICROORGANISMS
NUCLEIC ACID REPLICATION
PARASITES
RODENTS
SENSITIVITY
TUMOR CELLS
VERTEBRATES
VIRUSES