Restricted infectivity of ecotropic type C retroviruses in mouse teratocarcinoma cells: studies on viral DNA intermediates

Journal Article · Tue Jan 01 04:00:00 EST 1980 · J. Supramol. Struct.; (United States)

DOI:https://doi.org/10.1002/jss.400140211· OSTI ID:5679073

Yang, W K; d'Auriol, L; Yang, D M; Kiggans, Jr, J O; Ou, C; Peries, J; Emanoil-Ravicovitch, R

Replication of Gross strain N-tropic type C retrovirus was markedly restricted in a pluripotential undifferentiated embryonal cell line (PCC/sub 4/) of murine teratocarcinoma, whereas the same virus could cause productive infection in a myoblast-derived differentiated line (PCD/sub 1/) of the same tumor origin. To investigate the restriction mechanism, we compared the initial viral DNA formation in these two cell lines. Analyses by means of a modified Hirt extraction procedure and a modified Southern gel transfer method indicated that PCC/sub 4/ and PCD/sub 1/ cells supported the synthesis of viral DNA intermediates after inoculation of the Gross virus. In both cells a linear DNA duplex (form III viral DNA) appeared at 4 h, reached a maximal level at 8 to 9 h, and declined rapidly thereafter, while two closed-circular supercoiled DNA duplexes (form I viral DNA) showed their appearance, increase and decline in the 8 to 24 h period. During the period from 34 to 78 h after virus inoculation, another burst of viral DNA synthesis occurred in PCD/sub 1/ cells, presumably due to secondary virus infection, while at this period both form III and form I viral DNAs became undetectable in PCC/sub 4/ cells. The Hirt supernatant DNAs prepared from PCD/sub 1/ and PCC/sub 4/ cells 10 h after virus inoculation were equally infectious for NIH3T3 cells in a DNA transfection assay. Both PCD/sub 1/ and PCC/sub 4/ cells were very poor recipients for DNA transfection, although one positive result with PCD/sub 1/ cells might suggest a difference between the two cell types in this aspect. These results indicate that restriction of type C retrovirus in undifferentiated embryonal carcinoma cells occurs at a step subsequent to formation and maturation of viral DNA intermediates.

• Research Organization: Oak Ridge National Lab., TN
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 5679073
• Journal Information: J. Supramol. Struct.; (United States), Journal Name: J. Supramol. Struct.; (United States) Vol. 14; ISSN JSPMA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL PATHWAYS
BIOSYNTHESIS
CARCINOMAS
DISEASES
DNA
EMBRYONIC CELLS
INFECTIVITY
KINETICS
MICROORGANISMS
MOLECULAR BIOLOGY
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
REACTION KINETICS
STRUCTURAL CHEMICAL ANALYSIS
SYNTHESIS
VIRUSES