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Differential gene expression in Neurospora crassa cell types. Annual progress report, April 1, 1976--June 30, 1977

Technical Report ·
OSTI ID:7094388
During this period major emphasis was given on the new phase of this continued project work concerning studies of RNA-Dependent DNA polymerase (RDDP) activities in N. crassa cell types. RNA-Dependent DNA polymerase was partially purified and DNA products synthesized by RDDP were partially analyzed. Studies were initiated to detect RDDP activities in a few mutants of N. crassa and a few other filamentous fungi. Different rRNA molecules were isolated at different (NH/sub 4/)/sub 2/ SO/sub 4/ saturation levels and were characterized by gel-electrophoresis. A comparative study of RDDP activities using different sizes of RNA molecules confirmed our previous observations that size of template RNAs for RDDP activities was approximately 6S. rRNAs isolated from conidial and mycelial cells of N. crassa were hybridized with /sup 32/P-repeated and /sup 32/P-nonrepeated DNAs of mycelial cells. No significant difference in /sup 32/P-repeated DNA reactions when hybridized with rRNAs isolated from conidia and mycelia was noticed, whereas /sup 32/P-nonrepeated DNAs from mycelial cells, when hybridized with rRNAs from mycelial and conidial cells, showed slight variations, subject to further confirmations.
Research Organization:
Howard Univ., Washington, DC (USA). Dept. of Botany
DOE Contract Number:
EY-76-S-05-4182
OSTI ID:
7094388
Report Number(s):
TID-27688
Country of Publication:
United States
Language:
English

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Related Subjects

550200 -- Biochemistry
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGY
BIOMASS
CONIDIA
DAYS LIVING RADIOISOTOPES
DNA
ELECTROPHORESIS
ENERGY SOURCES
ENZYMES
FUNGI
GENES
GENETICS
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
METABOLISM
MUTATIONS
MYCELIUM
NEUROSPORA
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLANT TISSUES
PLANTS
POLYMERASES
RADIOISOTOPES
RENEWABLE ENERGY SOURCES
RNA
SPORES