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RNase-sensitive DNA polymerase activity in cell fractions and mutants of Neurospora crassa

Journal Article · · Biochem. Genet.; (United States)
DOI:https://doi.org/10.1007/BF00484590· OSTI ID:5856950
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RNase-sensitive DNA polymerase activity was tested in different cell fractions of Neurospora crassa cell types and its morphological mutants. This RSDP was found localized in the microsomal pellet fraction and absent in the purified nuclear pellets isolated from different N. crassa cell types: conidia, germinated conidia, and mycelia. This enzyme is capable of synthesizing a DNA product only in the presence of all four deoxyribonucleoside-5'-triphosphates and Mg/sup 2 +/. Removal of RNA from the pellet fraction by RNase strongly inhibited the DNA synthesis. The endogenous synthesis of DNA in the microsomal pellet fraction was associated with the formation of an RNA:DNA hybrid as analyzed by Cs/sub 2/SO/sub 4/ equilibrium density gradient centrifugation. The DNA product after alkali hydrolysis hybridizes with the RNA isolated from the same pellet fraction, as analyzed by elution from hydroxylapatite column at 60 C. This DNA product did not hybridize with poly(A). A few mutants tested showed this RNase-sensitive DNA polymerase activity.
Research Organization:
Howard Univ., Washington, DC
OSTI ID:
5856950
Journal Information:
Biochem. Genet.; (United States), Journal Name: Biochem. Genet.; (United States) Vol. 18:7/8; ISSN BIGEB
Country of Publication:
United States
Language:
English

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Related Subjects

550200 -- Biochemistry
550400 -- Genetics
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
CENTRIFUGATION
CHARGED PARTICLES
CHEMICAL ANALYSIS
COMPARATIVE EVALUATIONS
DNA
ENZYME ACTIVITY
ENZYMES
FUNGI
INHIBITION
IONS
MAGNESIUM IONS
MORPHOLOGY
MUTANTS
NEUROSPORA
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PLANTS
POLYMERASES
QUANTITATIVE CHEMICAL ANALYSIS
RNA
SEPARATION PROCESSES
TRANSFERASES
ULTRACENTRIFUGATION