Endogenous RNA-bound RNA-dependent DNA polymerase activity in Neurospora crassa

Journal Article · Sat Jan 01 04:00:00 EST 1977 · Exp. Mycol.; (United States)

DOI:https://doi.org/10.1016/S0147-5975(77)80016-9· OSTI ID:5043102

Dutta, S K ^[1]; Beljanski, M; Bourgarel, P

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1. Howard Univ., Washington, DC

Evidence indicating the presence of a RNA-bound RNA-dependent DNA polymerase (RDDP) in the eukaryotic fungus Neurospora crassa is presented. This enzyme, free of DNA-dependent DNA polymerase, is capable of synthesizing DNA only in the presence of all four deoxyribonucleoside 5'-triphosphates and Mg/sup 2 +/. The DNA polymerase activity is decreased considerably if the RNA bound to the enzyme is eliminated. The activity can be restored by addition of some particular RNA but not by addition of DNA. Both template RNA and the synthesized DNA product sedimented on sucrose density gradients at approximately 6 S. RNA-bound RNA-dependent DNA polymerase partially purified from extracts of N. crassa was distinguishable from known DNA-dependent DNA polymerases. DNA synthesized by RDDP was acid-precipitable and complementary to enzyme-bound RNA.

• OSTI ID: 5043102
• Journal Information: Exp. Mycol.; (United States), Journal Name: Exp. Mycol.; (United States) Vol. 1; ISSN EXMYD
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
BIOCHEMICAL REACTION KINETICS
CATALYSIS
CELL CULTURES
DNA
DNA REPLICATION
DNA-ASE
ENZYMES
ESTERASES
FUNGI
HYDROLASES
KINETICS
METABOLISM
NEUROSPORA
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHOSPHATASES
PLANTS
REACTION KINETICS
RNA