Autoimmunity induced by HgCl/sub 2/ in Brown-Norway rats. I. Production of monoclonal antibodies

Journal Article · Thu May 01 04:00:00 EDT 1986 · J. Immunol.; (United States)

OSTI ID:7094265

Hirsch, F; Kuhn, J; Ventura, M; Vial, M C; Fournie, G; Druet, P

Mercuric chloride (HgCl/sub 2/) induces in Brown-Norway rats (BN) a B cell polyclonal activation resulting in autoimmune diseases. Spleen cells from BN rats injected with HgCl/sub 2/ were fused with IR983F, a non-secreting rat myeloma cell line, in order to obtain monoclonal antibodies reacting with autoantigens or IgE-producing hybridomas. After screening for immunoglobulin-producing clones, the authors found 5% clones with anti-tissue activity, 8% with anti-TNP activity, and 41% secreting IgE. Among the anti-tissue monoclonal antibodies, one recognizes both TNP and mesangial structures of rat normal glomeruli, which could be an as yet unrecognized mechanism of nephrotoxicity. These experiments 1) confirm that HgCl/sub 2/ induces polyclonal activation, 2) show that the mercury model is of interest to obtain monoclonal IgE and various autoantibodies, and 3) suggest a new possible mechanism of antibody-mediated renal injury.

• Research Organization: INSERM, Paris, France
• OSTI ID: 7094265
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 136:9; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOLOGICAL EFFECTS
BODY
CHLORIDES
CHLORINE COMPOUNDS
DISEASES
GLOMERULI
HALIDES
HALOGEN COMPOUNDS
HYBRIDOMAS
IMMUNITY
KIDNEYS
MAMMALS
MERCURY CHLORIDES
MERCURY COMPOUNDS
MERCURY HALIDES
MONOCLONAL ANTIBODIES
NEPHRITIS
ORGANS
RATS
RODENTS
SOMATIC CELLS
SPLEEN CELLS
UROGENITAL SYSTEM DISEASES
VERTEBRATES