Autoimmunity induced by HgCl/sub 2/ in Brown-Norway rats. II. Monoclonal antibodies sharing specificities and idiotypes with mouse natural monoclonal antibodies

Journal Article · Thu May 01 04:00:00 EDT 1986 · J. Immunol.; (United States)

OSTI ID:7094252

Lymberi, P; Hirsch, F; Kuhn, J; Ternynck, T; Druet, P; Avrameas, S

Spleen cells derived from BN rats receiving HgCl/sub 2/ were fused with the nonsecreting rat myeloma cell line IR983F. Supernatants from immunoglobulin-secreting hybrids for antibody activity against actin, tubulin, autologous and heterologous myosin, myoglobin, dsDNA, peroxidase, and the haptens TNP, NIP, NNP, and NBrP were severed. Six monoclonal antibodies (mAb) were found to react with antigen(s) of the panel. At least three groups of antibody specificities were identified: 1) clones reacting with TNP (1 IgM, 1 Ige); 2) clones reacting with horseradish peroxidase (1 IgM); and 3) clones possessing widespread reactivity for several antigens as found for mouse natural autoantibodies (2 IgM, 1 IgE). The idiotypic (Id) determinants of the 59 mAb were also analyzed. All rat mAb that possessed widespread reactivities bore this Id. Similar studies were performed in sera from normal and mercury-stimulated rats. The results indicate 1) a role for HgCl/sub 2/ in the stimulation of natural antibodies producing cells and 2) the existence of interspecies cross-reactive Id among mouse and rat natural antibodies.

• Research Organization: Institut Pasteur, Paris, France
• OSTI ID: 7094252
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 136:9; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ACTIN
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOLOGICAL EFFECTS
CARBOXYLIC ACIDS
CHLORIDES
CHLORINE COMPOUNDS
ENZYMES
GLOBIN
GLOBULINS
HALIDES
HALOGEN COMPOUNDS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYBRIDOMAS
IMMUNITY
MAMMALS
MERCURY CHLORIDES
MERCURY COMPOUNDS
MERCURY HALIDES
MICE
MONOCLONAL ANTIBODIES
MYOGLOBIN
MYOSIN
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
PEROXIDASES
PIGMENTS
PORPHYRINS
PROTEINS
RATS
RODENTS
SOMATIC CELLS
SPLEEN CELLS
VERTEBRATES