Anti-idiotypic antibody in rat transplantation immunity. I. Production of anti-idiotypic antibody in animals repeatedly immunized with alloantigens

Journal Article · Sun Dec 01 04:00:00 EST 1974 · J. Immunol.; (United States)

OSTI ID:7296044

McKearn, T J; Stuart, F P; Fitch, F W

Lewis x Brown Norway (LBN) F/sub 1/ antiserum to an idiotype of Lewis anti-BN alloantibody (referred to as the Id-1 idiotype) was used to test a variety of Lewis alloimmune sera for the presence of Id-1. Several such Lewis sera raised by BN antigen injection at monthly intervals possessed the Id-1 idiotype. Hyperimmune Lewis anti-BN alloantiserum raised by six weekly injections of BN antigen, however, did not possess the Id-1 idiotype, but appeared to possess anti-idiotypic antibody to Id-1 instead. The anti-BN hemagglutinin titer, anti-idiotypic antibody titer to Id-1, and levels of the Id-1 idiotype were then measured in individual weekly serum samples from Lewis rats repeatedly immunized with BN antigen. The anti-BN hemagglutinin titers followed a cyclical pattern, with the Id-1 idiotype predominating only in the first cycle of the anti-BN hemagglutinin response. Anti-idiotypic antibody was demonstrable in five of eight repeatedly immunized rats, and in each of these five animals the appearance of anti-idiotypic antibody to Id-1 followed the clearance of Id-1 from the serum. Anti-idiotypic antibody may play a role in the regulation or control of an established immune response.

• Research Organization: Univ. of Chicago
• OSTI ID: 7296044
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 113:6; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

550600* -- Medicine
551000 -- Physiological Systems
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
AGGLUTININS
ANIMALS
ANTIBODIES
ANTIGEN-ANTIBODY REACTIONS
ANTIGENS
GRAFT-HOST REACTION
HEMAGGLUTININS
IMMUNE REACTIONS
IMMUNE SERUMS
IMMUNITY
IMMUNOLOGY
MAMMALS
RATS
RODENTS
TRANSPLANTS
VERTEBRATES