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Fibrin endothelial interaction increases pulmonary endothelial permeability in vitro

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:7092945
; ;

Fibrin has been implicated in the genesis of lung vascular injury. They examined the effect of adherence of fibrin to endothelial cells on endothelial permeability to protein. Bovine pulmonary arterial endothelial cells (EC) were grown to confluence on a gelatinized polycarbonate micropore filter and mounted in a chamber in which the luminal and abluminal media could be sampled. Fibrin was deposited on the endothelium by adding sheep fibrinogen (0.5 mg/ml) in Dulbecco's Modified Eagle Medium (DMEM) to the luminal surface of the endothelium, and then clotting the fibrinogen with 1 U/ml ..cap alpha..-thrombin. The fibrin was kept in contact with the endothelium for 3 hrs in an incubator at 37/sup 0/C plus 5% CO/sub 2/. The endothelial permeability to albumin was assessed by measurement of /sup 125/I-albumin clearance after removal of the fibrin clot from the endothelium. Therefore, fibrin contact with the endothelium independently induces an increase in endothelial permeability to albumin. The reversibility implies a transient change in the permeability dependent on the fibrin endothelial interaction.

Research Organization:
Albany Medical College, NY
OSTI ID:
7092945
Report Number(s):
CONF-8604222-
Journal Information:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:4; ISSN FEPRA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADHESION
ALBUMINS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CATTLE
CLEARANCE
COAGULANTS
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENDOTHELIUM
EXCRETION
FIBRIN
HEMATOLOGIC AGENTS
HEMOSTATICS
IN VITRO
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LUNG CLEARANCE
LUNGS
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PERMEABILITY
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RESPIRATORY SYSTEM
RUMINANTS
SCLEROPROTEINS
TISSUES
TRACER TECHNIQUES
VERTEBRATES