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Isoproterenol attenuates the thrombin-induced increase in pulmonary endothelial permeability

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5508387
;
The proposed permeability-decreasing effect of the beta-agonist, isoproterenol, was tested by measurement of the clearance rate of /sup 125/I-albumin across bovine pulmonary artery endothelial cell monolayers. These cells were seeded on gelatinized polycarbonate filters and mounted in a modified Boyden chamber with Dulbecco's Modified Eagle Medium (DMEM) and 1% BSA in the luminal and abluminal chambers. Baseline clearance rates were measured for an initial 30 min with the cells incubated at 37/sup 0/C in DMEM. At 30 min, 70 ul of either DMEM, ..cap alpha..-thrombin (10/sup -6/M), ..cap alpha..-thrombin and isoproterenol (10/sup -6/M) or isoproterenol alone was added to the luminal chamber. Clearance rates were determined at 30-60 min in each group and normalized to the baseline clearance rates of each (n = 8) cell monolayer. The experimental/baseline values were 0.98 +/- 0.08 (mean +/- SEM) for DMEM, 1.90 +/- 0.10 for thrombin, 1.52 +/- 0.08 for thrombin and isoproterenol, and 0.83 +/- 0.10 for isoproterenol alone. Thrombin increased (p < 0.05) the clearance rate from the control DMEM value and isoproterenol reduced (p < 0.05) the response to thrombin. Therefore, isoproterenol attenuates the thrombin-induced increase in pulmonary endothelial permeability.
Research Organization:
Albany Medical College, NY
OSTI ID:
5508387
Report Number(s):
CONF-8604222-
Conference Information:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
ANIMAL TISSUES
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
CLEARANCE
COAGULANTS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENDOTHELIUM
ENZYMES
HEMATOLOGIC AGENTS
HEMOSTATICS
HYDROLASES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LUNGS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HYDROLASES
PERMEABILITY
PROTEINS
RADIOISOTOPES
RESPIRATORY SYSTEM
SERINE PROTEINASES
SYMPATHOMIMETICS
THROMBIN
TISSUES
TRACER TECHNIQUES