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Transendothelial albumin flux: evidence against active transport of albumin

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5495245
; ; ;

The authors studied whether albumin is actively transported across cultured pulmonary endothelium by comparing the transendothelial flux of /sup 125/I-albumin from the luminal-to-abluminal side to the flux from the abluminal-to-luminal side. Bovine pulmonary artery endothelial cells were grown to confluence on gelatinized polycarbonated filters separating abluminal from luminal compartments. Each compartment had an albumin concentration of 1 g/100 ml to equalize oncotic pressure gradients. The effect of hydrostatic pressure was eliminated by maintaining an equal level of fluid in both compartments. The transendothelial flux of albumin across the monolayer was measured by placing /sup 125/I-albumin tracer either on the luminal or the abluminal side. Equal fluxes of /sup 125/I-albumin from luminal-to-abluminal side and from abluminal-to-luminal side were observed. The results indicate that the pulmonary endothelium behaves symmetrically for albumin, indicating the absence of active transport of albumin.

Research Organization:
Albany Medical College, NY
OSTI ID:
5495245
Report Number(s):
CONF-8604222-
Journal Information:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:3; ISSN FEPRA
Country of Publication:
United States
Language:
English

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Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CATTLE
CELL CULTURES
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
ENDOTHELIUM
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LUNGS
MAMMALS
MEMBRANE TRANSPORT
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PRESSURE GRADIENTS
PROTEINS
RADIOISOTOPES
RESPIRATORY SYSTEM
RUMINANTS
TISSUES
TRACER TECHNIQUES
VERTEBRATES