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Radioligand assay for angiotensin II receptors

Journal Article · · Methods Enzymol.; (United States)
; ; ;

An assay procedure for measurement of angiotensin II (AII) receptors with monoiodinated /sup 125/I-angiotensin II in partially purified adrenal membranes and isolated adrenal cells is described. The same general principles can be applied to the assay of receptors with (/sup 3/H)angiotensin II and radiolabeled agonist and antagonist analogs of the octapeptide. The Asp or Asn, and Val or Ile forms of AII, can be used for radioiodination to prepare tracer suitable for receptor binding studies. For some purposes, the Sar derivative of AII is preferable for labeling, since it has higher affinity for AII receptors and may provide higher specific binding of tracer when small numbers of receptor sites are to be analyzed.

OSTI ID:
7092482
Journal Information:
Methods Enzymol.; (United States), Journal Name: Methods Enzymol.; (United States) Vol. 109; ISSN MENZA
Country of Publication:
United States
Language:
English

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Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
400700 -- Radiochemistry & Nuclear Chemistry
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL GLANDS
ANGIOTENSIN
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BODY
CARDIOVASCULAR AGENTS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL REACTIONS
DATA
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENDOCRINE GLANDS
EXPERIMENTAL DATA
GLANDS
GLOBULINS
HALOGENATION
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINATION
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MEMBRANE PROTEINS
MEMBRANES
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
RADIORECEPTOR ASSAY
RECEPTORS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VASOCONSTRICTORS