Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Agonist-activated angiotensin II receptor is associated with a guanine nucleotide binding protein in bovine adrenal cortex

Journal Article · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6936699
; ;

Previous studies have shown that high affinity binding of the agonist Angiotensin II (Ang II) to adrenal cortex receptors was modulated by guanine nucleotides. In contrast, the antagonist (/sup 1/SAR,/sup 8/Ile) Ang II (SARILE) did not display any sensitivity toward guanine nucleotides. The authors have observed already after solubilization in octyl-glucoside and size-exclusion HPLC that agonist (/sup 131/I) (/sup 1/SAR) Ang II (SARANG)-stabilized receptor complex behaved as a larger protein than the antagonist (/sup 125/I)SARILE-stabilized receptor. Their purpose was to assay the direct association of a guanine nucleotide binding protein to the agonist-occupied Ang II receptor using (/sup 35/S) guanosine-thio-triphosphate (GTP-..gamma..-s) as a probe of the regulation protein. Adrenal cortical membrane receptors were activated with 10/sup -6/ M SARANG, solubilized and fraction marked on DEAE-agarose, then on size-exclusion HPLC. Fractionated samples were assayed with (/sup 35/S)-GTP-..gamma..-s. They found that a (/sup 35/S)GTP-..gamma..-s binding protein peak coeluted with the agonist-occupied receptor peak, while it disappeared in absence of agonist and eluted presumably free in a higher elution volume. Their results demonstrated directly the coupling of a guanine-nucleotide binding protein to the agonist-activated Ang II receptor.

Research Organization:
Clinical Research Institut of Montreal, Quebec
OSTI ID:
6936699
Report Number(s):
CONF-8604222-
Journal Information:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:4; ISSN FEPRA
Country of Publication:
United States
Language:
English

Similar Records

Characterization of cholate-solubilized dopamine D/sub 2/ receptors coupled to guanine nucleotide regulatory protein in bovine striatum
Conference · Tue Mar 04 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) · OSTI ID:6846421
Solubilization of a guanine nucleotide-sensitive form of the P2Y-purinergic receptor
Journal Article · Mon Jul 01 00:00:00 EDT 1991 · Molecular Pharmacology; (United States) · OSTI ID:5451645
Guanine nucleotide-binding protein regulation of melatonin receptors in lizard brain
Journal Article · Mon May 01 00:00:00 EDT 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA) · OSTI ID:5185879

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANGIOTENSIN
ANIMALS
AROMATICS
AZAARENES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
BRAIN
CARDIOVASCULAR AGENTS
CATTLE
CENTRAL NERVOUS SYSTEM
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
DRUGS
EVEN-ODD NUCLEI
GLOBULINS
GUANINE
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MEMBRANE PROTEINS
NERVOUS SYSTEM
NUCLEI
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
PURINES
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
RUMINANTS
SEPARATION PROCESSES
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES
VASOCONSTRICTORS
VERTEBRATES