Characterization of cholate-solubilized dopamine D/sub 2/ receptors coupled to guanine nucleotide regulatory protein in bovine striatum

Conference · Wed Mar 05 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6846421

Kazmi, S M.I.; Ramwani, J; Mishra, R K

Considerable evidence suggest that membrane-bound dopamine D/sub 2/ receptor exhibits high and low affinity states for agonists and the high affinity sites are coupled to guanine nucleotide regulatory protein (G-protein). Since discrepant findings were reported for the retention of functional dopamine - G-protein complexes following solubilization, they carried out the detailed characterization of cholic acid - NaCl solubilized receptors using (/sup 3/H)-spiperone, (/sup 3/H)-N-propylnorapomorphine (NPA) and (/sup 35/S)-GTP..gamma..S binding. The solubilized receptor not only retained all of its pharmacological specificities, but also exhibited high affinity (/sup 35/S)-GTP..gamma..S and (/sup 3/H)-NPA binding and the agonist (NPA) interaction was completely abolished by 100 ..mu..M Gpp(NH)p. Further, Gpp(NH)p significantly reduced the affinity of agonists in (/sup 3/H)-spiperone/agonists competition studies. When the solubilized preparation was subjected to DEAE-chromatography, the receptors eluted in two distinct peaks. Several evidence indicate that the two peaks represent different subpopulation of dopamine receptors with reference to high affinity agonist binding and sensitivity to guanine nucleotides. These findings suggest that the D/sub 2/ receptor-G-protein complex is retained following solubilization and the agonist high affinity component can be resolved chromatographically.

• Research Organization: McMaster Univ., Hamilton, Ontario
• OSTI ID: 6846421
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
AMINES
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZAARENES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CARDIOTONICS
CARDIOVASCULAR AGENTS
CATTLE
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
DOPAMINE
DRUGS
ELECTROPHORESIS
EVEN-ODD NUCLEI
GUANINE
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
MEMBRANE PROTEINS
NEUROREGULATORS
NUCLEI
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHENOLS
POLYPHENOLS
PROTEINS
PURIFICATION
PURINES
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
RUMINANTS
SEPARATION PROCESSES
SOLUBILITY
SULFUR 35
SULFUR ISOTOPES
SYMPATHOMIMETICS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES