Granulocytosis-inducing tumor inhibits the production of B lymphocytes in murine bone marrow

Journal Article · Sun Dec 01 04:00:00 EST 1985 · J. Immunol.; (United States)

OSTI ID:7092062

Fueloep, G; Lee, M Y; Rosse, C

Mice bearing a transplantable CE mammary carcinoma have been shown to have greatly augmented rates of neutrophil production coupled with a marked diminution of bone marrow lymphocytes. The objective the present study was to test whether the loss of lymphocytes, and especially of B cells, from the bone marrow and spleen of tumor-bearing animals was due to a reduced rate of cell production and if so, at what level this response was regulated. A modified /sup 3/H-TdR pulse and chase analysis was used to assess the rates of production of small lymphocytes and B cells at weekly intervals after CE tumor transplantation. Although pre-B cells appeared in the peripheral marrow (caudal vertebrae, metatarsal bones) and spleen of tumor-bearing mice, these cells could not compensate for the continued decrease in the numbers of more mature B cells. During the second and third weeks of tumor growth, a steady state appears to have been reached in B cell production, which was at a level approximately 10 times below that of normal. Because pre-B cells are normally maintained by a less mature precursor population (2), the initial disappearance of c..mu../sup +/s..mu../sup -/ cells suggests that the CE mammary carcinoma exerts its modulatory influence on primary B cell production by inhibiting or eliminating the cells that eventually feed into the pre-B compartment. The nature of the regulatory factors apparently secreted by the tumor and the more precise identity of the target cells are under investigation.

• Research Organization: Univ. of Washington School of Medicine, Seattle
• DOE Contract Number: AT06-79EV10270
• OSTI ID: 7092062
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 135:6; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE MARROW CELLS
CARCINOMAS
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
DISEASES
GRANULOMAS
HYDROGEN ISOTOPES
INHIBITION
ISOTOPES
LABELLED COMPOUNDS
LEUKOCYTES
LIGHT NUCLEI
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
SPLEEN
TRITIUM
VERTEBRATES
YEARS LIVING RADIOISOTOPES