Skip to main content
U.S. Department of Energy
Office of Scientific and Technical Information

Assay for albumin messenger RNA in an vitro protein synthesizing system from wheat germ

Journal Article · · Nucleic Acids Res.; (United States)
DOI:https://doi.org/10.1093/nar/3.6.1427· OSTI ID:7091679
The synthesis of serum albumin in an in vitro protein-synthesizing system from wheat germ stimulated with rat liver polysomal RNA is demonstrated by immunoprecipitation. The newly synthesized albumin has the same electrophoretic mobility as rat serum albumin. There is a linear increase in precursor incorporation into total protein and albumin with increasing RNA concentration. Potassium and magnesium optima for albumin synthesis are different from those for total protein synthesis.
Research Organization:
Univ. of California, San Francisco
OSTI ID:
7091679
Journal Information:
Nucleic Acids Res.; (United States), Journal Name: Nucleic Acids Res.; (United States) Vol. 3:6; ISSN NARHA
Country of Publication:
United States
Language:
English