Identification of albumin-synthesizing polysomes from mouse liver and a mouse hepatoma cell line

Journal Article · Mon May 09 04:00:00 EDT 1977 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(77)91676-X· OSTI ID:5193101

Brown, P C; Papaconstantinou, J

Albumin-synthesizing polysomes from mouse liver and mouse hepatoma cells in tissue culture have been localized on sucrose gradients with /sup 125/I-labeled antimouse serum albumin used as a marker. Competition studies show that the /sup 125/I-labeled antibody binds specifically to albumin-synthesizing polysomes from both tissues. The /sup 125/I-labeled polysomes from liver and hepatoma cells have identical sedimentation properties on sucrose gradients, which indicates that the polysomes range in size from 9 to 14 ribosomes. This is comparable in size to polysomes from rat liver and Morris hepatoma. One significant difference between these albumin-synthesizing polysomes is that those extracted from hepatoma cells bind 70 percent less antibody than equivalent amounts of polysomes from liver cells. Since the level of albumin synthesis in the hepatoma cells is comparable to the level of albumin synthesis in vivo, this difference in antibody-binding capacity is not likely to be due to differences in polysomal content, but appears to be a characteristic difference between hepatoma and normal mouse liver cells.

• Research Organization: Oak Ridge National Lab., TN
• OSTI ID: 5193101
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 76:1; ISSN BBRCA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BINDING ENERGY
BIOCHEMISTRY
BODY
CELL CONSTITUENTS
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ENERGY
GLANDS
HEPATOMAS
IN VIVO
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LIVER
MAMMALS
MICE
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PROTEINS
RADIOACTIVE MATERIALS
RADIOISOTOPES
RIBOSOMES
RODENTS
SYNTHESIS
TISSUE CULTURES
VERTEBRATES