Mouse albumin mRNA in liver and a hepatoma cell line
For studies on the regulation of serum protein synthesis, we isolated mouse albumin mRNA by direct immunoprecipitation of albumin-synthesizing polysomes and oligo(dT)-cellulose chromatography of albumin polysomal RNA. This albumin mRNA sediments at about 17 S, which corresponds to a molecular weight of approximately 6.5 x 10/sup 5/ or 2,000 nucleotides. DNA, complementary to albumin mRNA, was synthesized with avian myeloblastosis virus RNA-dependent DNA polymerase. Hybridization of the cDNA to its template mRNA displayed a melting temperature of 89/sup 0/C when analyzed by thermal elution from a hydroxylapatite column, indicating a high degree of fidelity of the base pairings. Data from the hybridization analyses and cell-free translation studies indicate that the albumin mRNA is about 80 to 85% pure. Quantitation of albumin mRNA in total cytoplasmic RNA revealed that mouse liver contains about 10-fold more albumin mRNA sequences than Hepa-2 cells, a permanent mouse hepatoma cell line that has maintained the capacity to synthesize albumin.
- Research Organization:
- Oak Ridge National Lab., TN
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 5127622
- Journal Information:
- J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 254:12; ISSN JBCHA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
ANIMALS
BIOCHEMISTRY
BIOSYNTHESIS
BODY
CELL CULTURES
CHEMISTRY
COMPARATIVE EVALUATIONS
DIGESTIVE SYSTEM
DISEASES
DNA
GLANDS
HEPATOMAS
LIVER
MAMMALS
MESSENGER-RNA
MICE
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RNA
RODENTS
SYNTHESIS
TRANSCRIPTION
VERTEBRATES