Detection of de novo synthesized form of microsomal cytochrome P-448 by autofluorography
Journal Article
·
· Bull. Exp. Biol. Med. (Engl. Transl.); (United States)
This paper suggests a simple and informative method of detection of de novo synthesized forms of microsomal cytochrome P-450. The method is based on autofluorography of the gel after electrophoretic fractionation of liver microsomal proteins of rats previously injected with inducers of components of the monooxygenase system, followed by C 14-leucine. From the results obtained by autofluorography it is shown that during induction of the monooxygenase system of rat liver with MCh and TCDD at least two forms of cytochromes of the P-448 type with mol. wt. of 53 and 56 kd were synthesized de novo.
- OSTI ID:
- 7091657
- Journal Information:
- Bull. Exp. Biol. Med. (Engl. Transl.); (United States), Journal Name: Bull. Exp. Biol. Med. (Engl. Transl.); (United States) Vol. 101:1; ISSN BEXBA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AROMATICS
AUTORADIOGRAPHY
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOMEDICAL RADIOGRAPHY
BIOSYNTHESIS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMISTRY
CYTOCHROME OXIDASE
CYTOCHROMES
DIAGNOSTIC TECHNIQUES
ELECTROPHORESIS
ENZYMES
FLUOROSCOPY
HAEM DEHYDROGENASES
HYDROCARBONS
LABELLED COMPOUNDS
LEUCINE
LIVER CELLS
MAMMALS
MEDICINE
METABOLISM
MICROSOMES
NUCLEAR MEDICINE
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
OXIDOREDUCTASES
PIGMENTS
POLYCYCLIC AROMATIC HYDROCARBONS
PROTEINS
RADIOLOGY
RATS
RODENTS
SOMATIC CELLS
SYNTHESIS
VERTEBRATES
XENOBIOTICS
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AROMATICS
AUTORADIOGRAPHY
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOMEDICAL RADIOGRAPHY
BIOSYNTHESIS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMISTRY
CYTOCHROME OXIDASE
CYTOCHROMES
DIAGNOSTIC TECHNIQUES
ELECTROPHORESIS
ENZYMES
FLUOROSCOPY
HAEM DEHYDROGENASES
HYDROCARBONS
LABELLED COMPOUNDS
LEUCINE
LIVER CELLS
MAMMALS
MEDICINE
METABOLISM
MICROSOMES
NUCLEAR MEDICINE
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
OXIDOREDUCTASES
PIGMENTS
POLYCYCLIC AROMATIC HYDROCARBONS
PROTEINS
RADIOLOGY
RATS
RODENTS
SOMATIC CELLS
SYNTHESIS
VERTEBRATES
XENOBIOTICS