Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Comparison of the peptide map and functional properties of monooxygenases induced by 3-methylcholanthrene and. beta. -naphthoflavone

Journal Article · · Biochemistry (Engl. Transl.); (United States)
OSTI ID:5933107
; ;
The similarity of the catalytic, spectral, electrophoretic, and immunochemical properties of microsomal cytochromes P-448 (molecular weight 56,000), synthesized de novo after administration of 3-methylcholanthrene and ..beta..-naphthoflavone to rats, was demonstrated. The identity of the peptide maps of the microsomal and isolated cytochrome P-448 is evidence of adequacy of the method of limited proteolysis for establishing the homogeneity and comparing the structure of the microsomal hemoproteins. The data obtained substantiate the approach for the study of the similarity and differences in the structure and enzymatic activity of various forms of monooxygenases without their preliminary isolation from the microsomal membrane.
Research Organization:
Institute of Clinical and Experimental Medicine, Novosibirsk, USSR
OSTI ID:
5933107
Journal Information:
Biochemistry (Engl. Transl.); (United States), Journal Name: Biochemistry (Engl. Transl.); (United States) Vol. 51:8; ISSN BIORA
Country of Publication:
United States
Language:
English

Similar Records

Biosynthesis of the general form of monooxygenase induced by xenobiotics of the methylcholanthrene type in the rat liver
Journal Article · Fri Oct 10 00:00:00 EDT 1986 · Biochemistry (Engl. Transl.); (United States) · OSTI ID:5726872
Detection of de novo synthesized form of microsomal cytochrome P-448 by autofluorography
Journal Article · Sun Jun 01 00:00:00 EDT 1986 · Bull. Exp. Biol. Med. (Engl. Transl.); (United States) · OSTI ID:7091657
Ontogenetic expression of polycyclic aromatic compound-inducible monooxygenase activities and forms of cytochrome P-450 in rabbit. Evidence for temporal control and organ specificity of two genetic regulatory systems
Journal Article · Sun Jul 10 00:00:00 EDT 1977 · J. Biol. Chem.; (United States) · OSTI ID:7323708

Related Subjects

3-METHYLCHOLANTHRENE
550301 -- Cytology-- Tracer Techniques
550501* -- Metabolism-- Tracer Techniques
560300 -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMALS
ANTIGEN-ANTIBODY REACTIONS
AROMATICS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CATALYTIC EFFECTS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHEMISTRY
COUNTING TECHNIQUES
CYTOCHROMES
DECOMPOSITION
ENZYME ACTIVITY
ENZYMES
FLAVENOIDS
FLAVONES
GLOBULINS
HYDROCARBONS
IMMUNE REACTIONS
IMMUNOASSAY
IMMUNOGLOBULINS
IMMUNOLOGY
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUCINE
MAMMALS
MICROSOMES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANOIDS
OXIDOREDUCTASES
OXYGENASES
PEPTIDES
PIGMENTS
POLYCYCLIC AROMATIC HYDROCARBONS
PROTEINS
PROTEOLYSIS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RATS
RODENTS
SCINTILLATION COUNTING
STRUCTURE-ACTIVITY RELATIONSHIPS
TRACER TECHNIQUES
VERTEBRATES
XENOBIOTICS