Biosynthesis of the general form of monooxygenase induced by xenobiotics of the methylcholanthrene type in the rat liver

Journal Article · Fri Oct 10 04:00:00 EDT 1986 · Biochemistry (Engl. Transl.); (United States)

OSTI ID:5726872

Tsyrlov, I B; Chasovnikova, O B; Grishanova, A Yu; Lyakhovich, V V

When five structurally different inducers of the MC type (polycyclic aromatic and heterocyclic hydrocarbons, chloro-derivatives of biphenyl and dibenzo-p-dioxin) are introduced into Wistar rats, de novo synthesis of two P-448 hemoproteins (M/sub r/ 56,000 and 53,000), differing in their functional and immunological parameters, occurs. A comparison of the catalytic and immunochemical properties of five cytochromes P-448 (M/sub r/ 56,000), the data of electrophoretic, proteolytic, and inhibitor analyses did not reveal any differences in the preparations compared, with the exception of TCDD-microsomes, where a low content of this form of cytochrome P-448 and high molecular activity of it with respect to 3,4-benzpyrene and 7-ethoxyresorufin were demonstrated. The results obtained do not support the hypothesis of the possibility of induced synthesis of such a variety of individual forms of monoxygenases as would correspond to the number of variants of the chemical structure of the xenobiotic inducers.

• Research Organization: Institute of Clinical and Experimental Medicine, Novosibrisk, USSR
• OSTI ID: 5726872
• Journal Information: Biochemistry (Engl. Transl.); (United States), Journal Name: Biochemistry (Engl. Transl.); (United States) Vol. 51:4; ISSN BIORA
• Country of Publication: United States
• Language: English

Related Subjects

3-METHYLCHOLANTHRENE
550301* -- Cytology-- Tracer Techniques
550501 -- Metabolism-- Tracer Techniques
560300 -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AROMATICS
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOSYNTHESIS
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CATALYTIC EFFECTS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHEMISTRY
COUNTING TECHNIQUES
CYTOCHEMISTRY
CYTOCHROMES
DECOMPOSITION
DIGESTIVE SYSTEM
DIOXIN
ELECTROPHORESIS
ENZYMES
FLAVENOIDS
FLAVONES
GLANDS
HETEROCYCLIC COMPOUNDS
HYDROCARBONS
HYDROXYLATION
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LEUCINE
LIVER
LIVER CELLS
MAMMALS
METABOLISM
MICROSOMES
ORGANIC ACIDS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANOIDS
ORGANS
OXIDOREDUCTASES
OXYGENASES
PIGMENTS
POLYCYCLIC AROMATIC HYDROCARBONS
PROTEINS
PROTEOLYSIS
RADIOASSAY
RATS
REACTION KINETICS
RODENTS
SCINTILLATION COUNTING
SOMATIC CELLS
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES
XENOBIOTICS