A multiple indicator, residue detection method for measurement of amino acid transport in skeletal muscle in vivo

Conference · Sun Jan 01 04:00:00 EST 1984 · J. Nucl. Med.; (United States)

OSTI ID:7091455

Bading, J R; Ekman, L G; Roh, M S; Gelbard, A; Brennan, M F

The authors' aim is to develop a method, applicable to man, for quantitative study of amino acid transport in skeletal muscle using tracer amino acids labeled with N-13 or C-11. Fasted, barbiturate-anesthetized dogs were catheterized for femoral artery injection. A mixture of N-13 L-glutamate (Glu) and Tc-99m HSA in 0.5-1.0 ml of saline was bolus injected, and radiolabel kinetics recorded over superficial thigh muscle with a collimated detector. Data for recirculation correction were simultaneously recorded over the contralateral site. The extracellular agent EDTA, labeled with In-113m, was used in the same manner to study molecular kinetics in the interstitial fluid. Regional tissue masses were measured with transmission scans, and regional blood plasma volumes were determined from equilibrium plasma concentrations and quantitative scans of HSA. Plasma mean transit times were computed from HSA kinetics (height/area method) and used with the plasma volume and tissue mass data to determine regional plasma flow/tissue mass. Values ranged from 2 to 22 ml/min/100g. Extraction efficiencies E were determined graphically from the kinetic data. Measured capillary E's for both Glu and EDTA are directly related to the plasma mean transmit times. The data indicate the capillary wall to be the primary barrier to passage of glutamate into muscle cells; the interstitium-intracellular E is nearly unity. Capillary permeability-surface area products were computed (Renkin-Crone model) to be 4.6 +- 0.9 and 2.1 +- 0.5 ml/min/100g for Glu and EDTA respectively. This relatively simple method provides reproducible values of transport parameters which are consistent with published values.

• Research Organization: Memorial Sloan-Kettering Cancer Center, New York, NY
• OSTI ID: 7091455
• Report Number(s): CONF-840619-
• Conference Information: Journal Name: J. Nucl. Med.; (United States) Journal Volume: 25:5
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD FLOW
BLOOD PLASMA
BODY FLUIDS
CARBON 11
CARBON ISOTOPES
CARBOXYLIC ACIDS
CHELATING AGENTS
COUNTING TECHNIQUES
DOGS
EDTA
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GLUTAMIC ACID
HOURS LIVING RADIOISOTOPES
IN VIVO
INDIUM 113
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MATERIALS
METABOLISM
MINUTES LIVING RADIOISOTOPES
MUSCLES
NITROGEN 13
NITROGEN ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
RADIOISOTOPE SCANNING
RADIOISOTOPES
STABLE ISOTOPES
TECHNETIUM 99
TECHNETIUM ISOTOPES
TRACER TECHNIQUES
VERTEBRATES
YEARS LIVING RADIOISOTOPES