Metabolism of sup 3 H- and sup 14 C-labeled glutamate, proline, and alanine in normal and adrenalectomized rats using different sites of tracer administration and sampling

Journal Article · Tue Aug 01 04:00:00 EDT 1989 · Metabolism, Clinical and Experimental; (USA)

DOI:https://doi.org/10.1016/0026-0495(89)90056-5· OSTI ID:5546444

Said, H M; Chenoweth, M; Dunn, A ^[1]

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1. Univ. of Southern California, Los Angeles (USA)

Alanine, glutamate and proline labeled with 14C and 3H were infused into fasted normal and adrenalectomized rats. Alanine was administered by the A-V mode (arterial administration-venous sampling), and glutamate and proline by both the A-V and V-A (venous administration-arterial sampling) modes. The kinetics of 14C alanine and 14C glutamate differed markedly from those of the tritium-labeled compounds, but there was little difference in the kinetics of 3H and 14C proline. The replacement rate calculated from the A-V mode for glutamate was about half that obtained in the V-A mode, but there was little difference with proline. The masses of the amino acids (total content of amino acids in the body) were calculated from the washout curves of the tritium-labeled compounds after the infusion of tracer was terminated. The masses for the normal rats were 407 mumol/kg for alanine, 578 mumol/kg for glutamate and 296 mumol/kg for proline. The so-called distribution spaces calculated conventionally from total masses and the amino acid concentrations in plasma are much greater than the volume of the body, reflecting the fact that amino acid concentrations in tissues greatly exceed those in plasma. Adrenalectomy markedly affected the kinetics of the three amino acids, and their replacement rates were greatly reduced. The proline and glutamate masses were reduced by at least one half, while that of alanine was unchanged. Adrenalectomy markedly reduced the conversion of proline to glutamate. The hydrocortisone regimen used in this study restored the metabolism of alanine and glutamate to normal, but had no effect on that of proline.

• OSTI ID: 5546444
• Journal Information: Metabolism, Clinical and Experimental; (USA), Journal Name: Metabolism, Clinical and Experimental; (USA) Vol. 38:8; ISSN METAA; ISSN 0026-0495
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ADRENALECTOMY
ALANINES
ALDEHYDES
AMINES
AMINO ACIDS
ANIMALS
ARTERIES
AZOLES
BLOOD VESSELS
BODY
BODY COMPOSITION
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
COMPARATIVE EVALUATIONS
CORTICOSTEROIDS
GLUCOCORTICOIDS
GLUCOSE
GLUTAMIC ACID
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HEXOSES
HYDROCORTISONE
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
LACTATES
MAMMALS
MEDICINE
METABOLISM
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PREGNANES
PROLINE
PYRROLES
PYRROLIDINES
RATS
RODENTS
SACCHARIDES
STEROIDS
SURGERY
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VEINS
VERTEBRATES